Molecular biological analysis in the process of carcinogenesis of hydatidiform mole cells transformed by tumor promoter agents

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The cultured cell line of molar cells employed in this study (BM-72) was driven from molar cysts obtained from a patient with complete hydatidiform mole. The carcinogenic promoter (TPA) was tested for the abillity to induce malignant transformation in cultured hydatidiform mole cells in terms of plating efficiency in double-layr soft agar and tumorigenesis. In testing for the effects of TPA,the cells were cultured in medium containing TPA (0.1,1.0,10,100 pg/ml) for 2 weeks. The tumorigenetic induction was seen following transplantation of 2 weeks TPA-treated cells into SCID mice and increase in tumorigenesis was noted in the 2-week treatment group with a response correlated to concentration. The technique of RAP-PCR differential display is useful for obtaining genes whose expression changes with various conditions. we have used the mRNA differential display method to identify genes whose expression is altered as a consequence of the transformation of BM-72 cultued cells treated by carcinogenic promoter, TPA (0.1,1.0,10,100 pg/ml), for 2 weeks. In this display, two clones were found to be not expressed in the cDNA of molar cells cultured in medium containing with 100 pg/ml of concentration of TPA in comparison to control and other concentration of TPA.