Molecular biological analysis in the process of carcinogenesis of hydatidiform mole cells transformed by tumor promoter agents

促癌剂转化葡萄胎细胞癌变过程的分子生物学分析

• 批准号: 07671825
• 负责人: KENJO Takiko
• 金额: $ 1.54万
• 依托单位: TOKAI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671825/
• 关键词: complete hydatidiform mole; cultured cells; SCID mice; RAP-PCR differential display; 絨毛性疾患; スキットマウス; 腫瘍形成; 発癌プロモーター; RT・PCR

The cultured cell line of molar cells employed in this study (BM-72) was driven from molar cysts obtained from a patient with complete hydatidiform mole. The carcinogenic promoter (TPA) was tested for the abillity to induce malignant transformation in cultured hydatidiform mole cells in terms of plating efficiency in double-layr soft agar and tumorigenesis. In testing for the effects of TPA,the cells were cultured in medium containing TPA (0.1,1.0,10,100 pg/ml) for 2 weeks. The tumorigenetic induction was seen following transplantation of 2 weeks TPA-treated cells into SCID mice and increase in tumorigenesis was noted in the 2-week treatment group with a response correlated to concentration. The technique of RAP-PCR differential display is useful for obtaining genes whose expression changes with various conditions. we have used the mRNA differential display method to identify genes whose expression is altered as a consequence of the transformation of BM-72 cultued cells treated by carcinogenic promoter, TPA (0.1,1.0,10,100 pg/ml), for 2 weeks. In this display, two clones were found to be not expressed in the cDNA of molar cells cultured in medium containing with 100 pg/ml of concentration of TPA in comparison to control and other concentration of TPA.

本研究中使用的磨牙细胞的培养细胞系（BM-72）是从完全性葡萄胎患者的磨牙囊肿中获得的。本文以体外培养的葡萄胎细胞为实验材料，通过双层软琼脂平板接种率和致瘤性试验，研究了致癌促进剂（TPA）对葡萄胎细胞恶性转化的诱导作用。在检测TPA的作用时，将细胞在含TPA（0.1、1.0、10、100 pg/ml）的培养基中培养2周。在将TPA处理的细胞移植到SCID小鼠中2周后观察到肿瘤发生诱导，并且在2周处理组中注意到肿瘤发生增加，其中响应与浓度相关。RAP-PCR差异显示技术可用于获得表达随不同条件而变化的基因。我们已经使用mRNA差异显示方法来鉴定其表达由于用致癌启动子TPA（0.1、1.0、10、100 pg/ml）处理BM-72培养细胞2周而改变的基因。在该展示中，发现与对照和其它浓度的TPA相比，在含有100 pg/ml浓度的TPA的培养基中培养的磨牙细胞的cDNA中不表达两个克隆。

项目成果

見常多喜子: "新しく樹立した全胞状奇胎由来培養細胞のSCIDマウスへの移植" 日本癌学会総会記事. 100 (1995)

Takiko Mitsune：“将新建立的完整葡萄胎来源的培养细胞移植到 SCID 小鼠中”日本癌症协会大会文章 100 (1995)。

見常多喜子: "新しく樹立した全胞状奇胎由来培養細胞のSCIDマウスへの移植" 日本癌学会総会記事(第54回総会). 100- (1995)

Takiko Mitsune：“将新建立的完整葡萄胎来源的培养细胞移植到 SCID 小鼠中”日本癌症协会大会文章（第 54 届大会）（1995 年）。

見常多喜子: "全胞状奇胎組織、培養細胞のSCIDマウスへの移植" 日本産科婦人科学会誌. 48. 487 (1996)

Takiko Mitsune：“将整个葡萄胎组织和培养细胞移植到 SCID 小鼠中”日本妇产科学会杂志 48. 487 (1996)。

見常多喜子: "新しく樹立した胞状奇胎由来培養細胞の諸性格" Human Cell. 7(2). 39 (1994)

Takiko Mitsune：“源自葡萄胎的新培养细胞的特征”Human Cell 7(2) 39 (1994)。

TAKIKO KENJO: "Transplantation to SCID mice in the tissue of complete hydatidiform mole and molar cultured cells" ACTA OBSTETRICA ET GYNAECOLOGICA. 48, Sup.487 (1996)

TAKIKO KENJO：“将完全葡萄胎和磨牙培养细胞的组织移植到 SCID 小鼠”《妇产学学报》。