Serial section reconstruction of the guinea pig outer and inner hair cells as studied with a high-voltage electron microscope.

用高压电子显微镜研究豚鼠外毛细胞和内毛细胞的连续切片重建。

• 批准号: 62570774
• 负责人: SHINKAWA Hideichi
• 金额: $ 1.15万
• 依托单位: Hirosaki University (1989)Tohoku University (1987-1988)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570774/
• 关键词: High-voltage electron microscope; Guinea pig; Cochlea; Outer hair cell; Inner hair cell; Afferent nerve terminal; Efferent nerve terminal

The fine structural arrangement of neural terminals on outer and inner hair cells of guinea pig was investigated by thick consecutive sectioning techniques. Specimens collected on single hole grids were observed under a high-voltage electron microscope.Complete serial sections from 89 outer hair cells(OHCs) were available for the present study. Both afferent and efferent neural terminals on each OHC varied in number from 5 to 35. Minimum and maximum values were obtained at the hook portion and at the second and third rows of the third turn,respectively. As regarding the afferent nerve, numerous endings were seen in the second and third rows of the second and third turns. In contrast with afferent terminals, efferent endings showed less remarkable changes in number from the basal end to the apex, ranging from 1 to 11. OHCs in the first row possessed maximum innervation density of efferents at the upper part of the second and third turns. On the other hand, those in the second and third rows had maximum number of endings in the basal turn. Longest diameters of the afferent nerve terminals were measured, varying from 500 to 3300 nm. Most of endings ranged in size from 600 to 1700nm.Consecutive sections from 27 inner hair cells (IHCs) were observed in the present experiment. Both afferent and efferent nerve endings on each IHC varied in number from 8 to 24 and 4 to 7, respectively. IHCs in the basal turn was most densely innervated by afferent nerve terminals. Efferent endings showed no remarkable change in number from the hook portion to the apex. These endings rarely formed synapses with IHCs. Many of the radial afferent fibers examined contacted single IHCs with single terminal swellings. Among IHCs, 60% of the afferent synapses possessed presynaptic bodies.

用厚连续切片技术研究了豚鼠内、外毛细胞神经末梢的精细结构排列。在单孔网格上收集的标本在高压电子显微镜下观察。本研究获得了89个外毛细胞的完整连续切片。每个OHC上的传入和传出神经末梢的数目从5到35个不等。最小和最大值分别出现在钩部和第三圈的第二排和第三排。至于传入神经，在第二和第三个转弯的第二和第三排可见大量的末梢。与传入末梢相比，传出末梢的数量从基端到顶端变化不大，从1到11个不等。第一排的神经末梢在第二、三圈上部的传出神经分布密度最大。另一方面，排在第二和第三排的人在基本转折中有最多的结束次数。测量传入神经末梢的最大直径，范围为500~3300 nm。本实验观察了27个内毛细胞(IHC)的连续切片。每个IHC上的传入和传出神经末梢数目分别为8~24个和4~7个。基底弯的IHC由传入神经末梢最密集地支配。从钩部到心尖，传出末梢的数目没有明显变化。这些终末很少与IHC形成突触。许多被检查的放射状传入纤维与单端肿胀的单个IHC接触。其中，60%的传入突触具有突触前小体。