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Ultramicroscopic Study on the Vestibular Ganglion and Distribution of the Afferent Vestibular Nerve

前庭神经节及传入前庭神经分布的超显微研究

基本信息

批准号：
62570778
负责人：
TAGASHIRA Nobuharu
金额：
$ 1.6万
依托单位：
Hiroshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

项目摘要

The fine structures of the vestibular ganglion of the frog and the guinea pig were studied three-dimensionally using the scanning, transmission electron and light microscopy.The present study was based upon our previous results that ototoxic drugs and bacteria (Mycobacterium fortuitum) induce morphological changes in the vestibular sensory epithelia and the vestibular ganglion of the guinea pig and the mouse. Furthermore, the study revealed that both ototoxic drugs and bacteria have direct destructive effect not only on the sensory epithelia but also on the vestibular ganglion cells including neuronal cell and Schwann cell. The running course of the afferent nerve fibers from each vestibular receptor to the vestibular ganglion and the distributions of the neuronal cells from each receptor in the ganglion were examined using horseradish peroxidase (HRP) method. In this study, exact course of the afferent nerve fibers and distribution of the neuronal cells could not be clarified, because … More we applied HRP on the live experimental animals (bull frog). However, we detected several neuronal cells stained with HRP when the lateral ampullary nerve was treated. We expect that the technique will be improved so that HRP could be iontophoretically injected into an identified axon of each receptor. This will clarify connection between the receptor, nerve fiber and neuronal cells.Other results are as follows; 1) The morphological changes of the vestibular sensory cepithelia of the guinea pig following electrical stimulation were examined. The direct stimulation at intensity of 3OOuA caused severe damages in the inner ear. 2) Effect of Mycobacterium fortuitum in the inner ear was investigated. Morphological changes of inner ear were studied in comparison with behavioral changes. 3) The ultrastructure of glycocalyx and ciliary interconnections of the vestibular sensory cells were investigated by the use of tannic acid and ruthenium red staining techniques. As a results, ciliary interconnections may keep the cilia arranged in a bundle and the tip links may be involved in sensory cell mechanical transduction system. Less

本研究应用扫描、透射电镜和光学显微镜对豚鼠和蛙前庭神经节的微细结构进行了三维立体观察，并在此基础上对耳毒性药物和细菌（偶发分枝杆菌）引起的豚鼠和小鼠前庭感觉上皮和前庭神经节的形态学变化进行了研究。此外，研究还发现，耳毒性药物和细菌不仅对感觉上皮细胞有直接的破坏作用，而且对前庭神经节细胞包括神经元细胞和雪旺细胞也有直接的破坏作用。用辣根过氧化物酶（HRP）法观察前庭各感受器传入神经纤维向前庭神经节的走行及神经元在前庭神经节内的分布。在这项研究中，传入神经纤维的确切路线和神经元细胞的分布无法澄清，因为 ...更多信息我们将HRP应用于活体实验动物（牛蛙）。然而，我们发现了几个神经元细胞的HRP染色时，外侧壶腹神经处理。我们希望这项技术将得到改进，使HRP可以离子电渗注入到一个确定的轴突的每一个受体。本实验还观察了电刺激豚鼠前庭感觉上皮细胞的形态学变化。强度为300uA的直接刺激导致内耳严重损伤。2)研究了偶然分枝杆菌在内耳中的作用。观察内耳形态学变化，并与行为学变化进行比较。3)用单宁酸和钌红染色法观察前庭感觉细胞的糖萼和纤毛连接的超微结构。因此，纤毛的相互连接可以使纤毛保持成束排列，并且末端连接可能参与感觉细胞的机械转导系统。少