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Electron-microscope study of changes and calcification in dental matrix with experimentally induced formative disturbances

实验诱导的形成障碍下牙基质变化和钙化的电子显微镜研究

基本信息

批准号：
62570816
负责人：
MIAKE Yasuo
金额：
$ 1.22万
依托单位：
Tokyo Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

1. Disturbance in dentin formation (1) Vinblastine administration: within the apical predentin, segmental collagen aggregates 320nm in length and 200nm in width with a structure consisting of numerous symmetrical bands were observed. With the passing of time, this abnormal collagen shifted towards and was incorporated into the dentin. (2) Sodium fluouide administration: One hour after NaF adminisration, segmental collagen aggreagtes and a large amount of an amouphous material containing a substance positively stainable to ruthnium red (acid-mucopolysaccharide) appeard. Six hours after administration, fibrous long-spacing collagens with 160nm periodic striation were observed. When they approached the calcification front, these two abnormal kinds of collagen were calcified as a result of crystal deposition. Observation of decalified sections revealed a fine linear structure in the abnormal collagen and slack connections among collagen molecules.2. Disturbance in enamel formation (1) Vinblastine administration: Forming enamel crystals were more slender and the enamel demonstrated a lower degree of calcification 24 hours after administration. Five days after administration, the surface of mature enamel was irreglar, and the enamel contained large amounts of organic matrix and cell fragments. (2) Sodium fluoride administration: Enamel-rod structure in the forming enamel was indistinct and an abnormal enamelwith slender crystals had formed 24 hours after administration.

1.牙本质形成障碍（1）长春碱组：在根尖前牙本质内可见长约320nm、宽约200nm的节段性胶原聚集体，其结构由许多对称的条带组成。随着时间的推移，这种异常的胶原蛋白向牙本质转移并被纳入牙本质中。(2)氟化钠给药：NaF给药后1小时，节段性胶原聚集，并出现大量含有正性粘多糖物质的无定形物质。给药后6h，观察到具有160nm周期性条纹的纤维状长间距胶原。当它们接近钙化前沿时，这两种异常胶原蛋白因晶体沉积而发生钙化。脱钙切片观察发现，异常胶原呈精细的线状结构，胶原分子间连接松弛.釉质形成障碍（1）长春碱给药：给药后24小时，形成的釉质晶体更细长，釉质钙化程度较低。给药后5天，成熟釉质表面不规则，釉质中含有大量的有机基质和细胞碎片。(2)氟化钠给药：给药后24小时，形成釉质中的釉质棒结构不清楚，并形成具有细长晶体的异常釉质。