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Acid mucopolysaccharide metabolism and bone formation in fish

鱼类酸性粘多糖代谢与骨形成

基本信息

批准号：
02660213
负责人：
SATO Mamoru
金额：
$ 1.34万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660213/
关键词：
rainbow trout ascorbic acid deficiency vertebra acid mucopolysaccharide chondroitin sulfate Chondroitin skeletal deformity hyaluronic acid 脊椎骨骨格形成篩骨

项目摘要

1)Acid mucopolysaccharides were extracted from the ethmoid bone and vertebra of normal rainbow trout, and determined by CPC-cellulose column chromatography, electrophoresis on cellulose acetate and. chondroitinase digestion. Chondroitin and hyaluronic acid were the major components of acid mucopolysaccharides in the ethmoid bone. Chondroitin sulfates were the major acid mucopolysaccharides in the vertebra.2)Rainbow trout weighing 0.3g on average were fed ascorbic acid(AsA)-deficient and AsAsupplemented diet for 15 weeks. The fish of AsA-deficient group began to show the typical signs of scurvy, such as lordosis and scoliosis, at the 12th week of rearing. The whole body acid mucopolysaccharides contents were considerably higher in the AsA-deficient group than in the AsA-supplemented-group. The contents of non-sulfated acid mucopolysaccharides decreased in the AsA-supplemented group with age but not decreased in the AsA-deficient group. The vertebral acid mucopolysaccharides contents in both groups were the same level before they developed the apparent signs of scurvy, whereas the contents in the AsA-deficient group increased extremely in the advanced stages of scurvy. There were no difference between both groups in the content ratios of chodroitin 4-sulfate and 6-sulfate of the acid mucopolysaccharides. However, the contents of chondroitin and hyaluronic acid of AsA-deficient group were higher than those of AsA-supplemented group.

1)从正常虹鳟鱼筛骨和脊椎骨中提取酸性粘多糖，采用CPC-纤维素柱层析、醋酸纤维素电泳和凝胶电泳法测定其含量。软骨素酶消化。软骨素和透明质酸是筛骨酸性粘多糖的主要成分。硫酸软骨素是脊椎中主要的酸性粘多糖。2）以平均体重为0.3g的虹鳟鱼为试验对象，分别饲喂抗坏血酸（阿萨）缺乏和添加AsA的饲料15周。AsA缺乏组的鱼在第12周开始出现典型的坏血病症状，如脊柱前凸和脊柱侧凸。AsA缺乏组大鼠全身酸性粘多糖含量显著高于AsA补充组。非硫酸化酸性粘多糖的含量在补充AsA组随年龄的增长而下降，但在AsA缺乏组不下降。两组动物在出现坏血病症状前脊椎酸粘多糖含量基本相同，而AsA缺乏组在坏血病晚期脊椎酸粘多糖含量显著升高。酸性粘多糖中4-硫酸软骨素和6-硫酸软骨素的含量比值两组间无差异。但AsA缺乏组软骨素和透明质酸含量高于AsA补充组。