Homeostatic maintenance of mineralization in dental hard tissue by saliva

这项研究旨在从结晶学角度阐明唾液或再矿化液在脱矿牙本质中的作用，并确定一种食品添加剂在牙周病治疗期间促进根部牙本质耐酸性的有效性。我们使用了20至40岁患者出于治疗原因拔除的完全阻生第三磨牙。拔除的牙齿的试验性使用获得了知情同意。东京牙科大学伦理委员会批准了材料的获取和实验使用。脱矿牙本质的再矿化通过唾液再矿化似乎是很好的，因为表面形成了一层再矿化层，再矿化在整个过程中都得到了改善。但高分辨透射电子显微镜显示，表层致密的晶体沉积实际上可能会抑制矿物的渗透到较低的水平。然而，在唾液中添加F-Ca-CO会导致矿化，不是在表面，而是在更深的…更多的矿层，因此被认为在长期内促进了整体矿化。再矿化溶液引起的深部矿化程度被认为是轻微的，因为溶液中的Ca和P含量较低。人们认为，添加F-Ca-CO会通过增加Ca和添加F而导致高度的再矿化。单独添加F会在表层引起强烈的再矿化，而F-Ca-CO的添加会导致脱矿层内区的再矿化。这一现象导致了这样一种想法，即F以外的东西起着很大的作用。牙本质抗脱矿试验浸泡在再矿化液中可增加牙本质对脱矿的抵抗力，添加F-Ca-CO会进一步增加它。由于钙和氟的作用导致更大的矿物沉积，添加剂改善了晶体的形成。因此，F-Ca-CO的加入被认为增强了对脱矿的抵抗。较少

This research was to clarify crystallographically the role of saliva or a remineralizing solution in demineralized dentin and to determine the effectiveness of a food additive to promote acid resistance in root dentin exposed during periodontal-disease therapy.We used completely impacted human third molars extracted for therapeutic reasons from patients aged from 20 to 40 yr. Informed consent was obtained for the experimental use of the extracted teeth. The Ethics Committee of Tokyo Dental College approved both the acquisition and the experimental use of the materials.1. Remineralization of demineralized dentinRemineralization by means of saliva would seem to be excellent because a remineralized layer forms on the surface and remineralization throughout improves. But HR-TEM shows that dense crystal deposition at the surface layer may actually inhibit mineral permeation to lower levels. The addition of F-Ca-CO to saliva, however, causes mineralization, not at the surface, but in deeper … More layers and therefore is thought to promote overall mineralization in the long term. The degree of deep-layer mineralization induced by a remineralizing solution is thought to be slight because the solution is low in Ca and P. It was believed that the addition of F-Ca-CO would cause high-degree remineralization by increasing Ca and adding F. The addition of F alone caused strong remineralization at the surface layer, whereas the addition of F-Ca-CO caused remineralization from the inner regions of the demineralized layer. This phenomenon led to the idea that something other than F plays a big role.2. Tests on Dentinal Demineralization ResistanceImmersion in a remineralizing solution increased dentinal resistance to demineralization, and the addition of F-Ca-CO increased it still further. Because it causes greater mineral deposition owing to the actions of Ca and F, the additive improves crystal formation. Consequently, the addition of F-Ca-CO is believed to strengthen resistance to demineralization. Less