The effct of Pulsed electromagnetic fields on proliferation and differentiation of rabbit costal chondrocytes in culture

先前的研究表明，脉冲电磁场（PEMF）刺激增强了兔肋软骨细胞原代培养中甲状旁腺激素（PTH）对cAMP、鸟氨酸脱羧酶和葡萄糖氨基聚糖合成的刺激。这表明，PEMF刺激增加了培养软骨细胞对甲状旁腺激素的敏感性，并促进了它们的分化。然而，PEMF对软骨细胞增殖的影响很小。PEMF刺激仅对甲状旁腺有影响。在软骨衍生因子、成纤维细胞生长因子、p物质和胰岛素样生长因子存在的情况下，无明显影响。本研究以正常大鼠肾细胞为实验对象，研究了脉冲电场刺激对细胞增殖的影响。在0.3% FCS存在的情况下，PEF刺激导致<@D13@> d3h -胸腺嘧啶入DNA增加30%。FCS浓度进一步升高，细胞增殖加速，PET刺激作用减弱。此外，PEF和PEMF刺激影响了软骨细胞小球培养钙化过程中的碱性磷酸酶活性。

Previous studies have shown that pulsed electromagnetic field (PEMF) stimulation potentiates parathyroid hormone(PTH) stimulations of cAMP, ornithine decarboxylase and glucosaminoglycan syntheses in primary cultures of rabbit costal chondrocytes.This indicates that PEMF stimulation angments the sensitivity of cultured cartilage cells to PTH and promotes their differentiation. However, PEMF had little effect on chondrocyte proliferation. The effect of PEMF stimulation was observed only in PTH. It did not give any effects in the presence of cartilage derived factor, fibroblast growth factor, substance-P, and insulin-like growth factor. In the present study, the effect of pulsed electric field(PEF) stimulation on cell proliferation was study using Normal rat kidney cells. PEF stimulation caused 30% increase in the incorporation of <@D13@>D3H-thymidine into DNA in the presence of 0.3% FCS. Further increase in the concentration of FCS accelerated the proliferation of cells and diminished the effect of the PET stimulation. Furthermore the PEF and PEMF stimulations affected alkaline phosphatase activity during the process of calcification in pellet cultures of chondrocytes.