Immunohistochemical investigation on the effect of the bone resorbint factors

骨吸收因子影响的免疫组织化学研究

• 批准号: 62570859
• 负责人: YOSHIMINE Yoshito
• 金额: $ 1.28万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570859/
• 关键词: Interleukin 1; Immunohistochemistry; ABC法; 骨吸収促進因子; 根尖病巣

The localization of the cytokine interleukin-1beta was studied immunohistochemically im human and rat periapical lesions.Human periapical lesions obtained by the apicoectomy were fixed with PLP fixative for 5 hours. Tat perispical sites were placed in 4% PFA and decalcified in 10% EDTA solution for 10 days.For the purpose of staining immunohistochemically for IL-1beta, we followed an avidin labelled biotin method with the use of peroxidase and alkaline phosphatase. In this mothod, sections were incubated for 2 hours, at room temperature, with the antibody (anti IL-1beta mouse monoclonal antibody against human recombinant IL-1beta).Several cell types were found to display positive immunoreactivity for IL-1beta. Cell types which stained particularly dark for UL-1beta were neutrophils, mononucleated cells, endothelial cells and osteoclasts.In conclusion, this investigation has demonstrated that interleukin is present in cells infiltrating into periapical lesions. Hence, this cytokine may be involved in the resorption of alveolar bone.

用免疫组织化学方法研究了细胞因子白细胞介素-1 β（IL-1 β）在人和大鼠根尖周病变中的定位。将达特细胞置于4%PFA中，10%EDTA溶液中脱钙10天，用过氧化物酶和碱性磷酸酶标记抗生物素蛋白生物素法对IL-1 β进行免疫组化染色。用抗人重组IL-1 β鼠单克隆抗体（antiIL-1 β mouse monoclonal antibody against human recombinant IL-1 β），在室温下孵育2 h，发现几种细胞类型显示IL-1 β免疫反应阳性。UL-1 β染色特别深的细胞类型是中性粒细胞、单核细胞、内皮细胞和破骨细胞。总之，这项研究表明，白细胞介素存在于细胞浸润到根尖周病变。因此，这种细胞因子可能参与牙槽骨的吸收。