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In situ expression of RANKL, RANK, osteoprotegerin and cytokines in osteoclasts of rat periodontal tissue

大鼠牙周组织破骨细胞中RANKL、RANK、骨保护素和细胞因子的原位表达

基本信息

批准号：
14571817
负责人：
YOSHIMINE Yoshito
金额：
$ 2.24万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571817/
关键词：
RANKL RANK osteoprotegerin osteoclast ISH cytokine periodontal tissue rat 歯根膜 M-CSF 蛍光抗体法破骨細胞

项目摘要

This study examined the in situ expression of RANKL, RANK, OPG, IL-1β and TNFα in the osteoclasts of rat periodohtal tissue. Four-week-old Wistar rats were used. Tooth movement was performed by the Waldo method, and the pathological bone resorption was induced. The mineralized maxillae and mandiblae were embedded with paraffin. In situ hybridization was performed to detect RANKL, RANK. OPG, IL-1β and TNFαmRNA in osteoclasts and other cells using the specific RNA probes, respectively. Both RANKL and RANK were concomitantly expressed in some osteoclasts. RANKL was also positive in osteoblasts and PDLs. No IL-1β and TNFα-positive osteoclast was noted. The positive signals of osteoprotegerin were detected in almost all osteoblasts, PDLs and odontoblasts. No osteoprotegerin-positive osteoclasts were observed. The number and the distribution pattern of RANKL-and RANK-expressing osteoclasts changed when orthodontic excessive force was applied to periodontal tissue. In addition, IL-1β and TNFα were shown to be expressed in osteoclasts under pathological status. These findings suggest that an autocrine mechanism of RANKL-RANK exists in osteoclast, which is heightened in the pathological conditions. Furthermore, the autocrine mechanism of IL-1β and TNFαis also provided in osteoclast under pathological condition. These autocrine mechanisms therefore seem to regulate the osteoclast function in both physiological arid pathological conditions.

本研究观察了RANKL、RANK、OPG、IL-1β和TNFα在大鼠牙周膜破骨细胞中的原位表达。使用4周龄Wistar大鼠。采用Waldo法进行牙齿移动，诱导病理性骨吸收。将矿化的上颌骨和下颌骨用石蜡包埋。原位杂交检测RANKL、RANK。用特异性RNA探针分别检测破骨细胞和其它细胞中OPG、IL-1β和TNFαmRNA的表达。RANKL和RANK在一些破骨细胞中同时表达。RANKL在成骨细胞和牙周膜细胞中也呈阳性表达。未观察到IL-1β和TNFα阳性破骨细胞。骨保护素在几乎所有成骨细胞、牙周膜细胞和成牙本质细胞中均呈阳性信号。未观察到骨保护素阳性破骨细胞。当正畸力作用于牙周组织时，表达RANKL和RANK的破骨细胞的数量和分布模式发生变化。病理状态下破骨细胞表达IL-1β和TNFα。这些结果表明，RANKL-RANK的自分泌机制存在于破骨细胞中，在病理条件下增强。此外，破骨细胞在病理状态下也存在IL-1β和TNFα的自分泌机制。因此，这些自分泌机制似乎在生理和病理条件下调节破骨细胞的功能。