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Histological studies on the biocompatibility of tetracalcium phosphate-based cement

磷酸四钙骨水泥生物相容性的组织学研究

基本信息

批准号：
06671919
负责人：
YOSHIMINE Yoshito
金额：
$ 0.26万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

1.Histologic healing processes were observed at 1,3,7, and 10 days after application with either tetracalcium phosphate cement or calcium hydroxide cement to the exposed pulp of the rat maxillary incisors. In teeth applied with calcium hydroxide cement, necrotic tissue was present beneath the cement before new hard tissue formed. In contrast, tetracalcium phosphate cement elicited a dentine formation with no evidence of either intervening tissue necrosis or marked inflammation. Furthermore on ultrastructural examination the newly formed hard tissue was in direct contact with the material. This study suggests that 4CP cement possesses a biocompatible property, which indicates its potential for use as a direct pulp-capping agent.2.Newly-developed tetracalcium phosphate-based cement (4CP cement) and cells derived from neonatal rat calvaria were cocultured to study the in vitro reaction of osteoblastic cells to the biomaterial at the light and electron microscopic levels. Three-dimensional nodular structures covered with active osteoblastic cells were formed in the periphery of the test material and they contained a mineralized tissue that exhibited features closely resembling bone formed in vivo. Ultrastructurally, the test material was circumscribed with an electron-dense structure, and was immediately adjacent to elongated cytoplasmic processes with intact morphology or collagen fibrils with periodic structures. Furthermore, the mineralization of the extracellular collagenous matrix occurred directly on the surface of the material. These in vitro findings suggest the propery of 4CP cement to bind directly with newly-formed hard tissues.

1.大鼠上颌切牙外露牙髓涂抹磷酸四钙骨水泥或氢氧化钙骨水泥后1、3、7、10天观察组织学愈合过程。在使用氢氧化钙水泥的牙齿中，在新的硬组织形成之前，坏死组织存在于水泥下方。相比之下，磷酸四钙水泥引起牙本质形成，没有任何介入组织坏死或明显炎症的证据。此外，在超微结构检查中，新形成的硬组织与材料直接接触。本研究表明4CP水门汀具有生物相容性，这表明其作为直接盖髓剂的潜力。2.将新开发的磷酸四钙基水门汀（4CP水门汀）与新生大鼠颅骨来源的细胞共培养，在光和电镜水平上研究成骨细胞对生物材料的体外反应。在测试材料的外围形成了覆盖有活性成骨细胞的三维结节结构，并且它们包含矿化组织，其表现出与体内形成的骨非常相似的特征。从超微结构上看，测试材料被电子致密结构包围，并紧邻具有完整形态的细长细胞质突起或具有周期性结构的胶原原纤维。此外，细胞外胶原基质的矿化直接发生在材料的表面上。这些体外研究结果表明 4CP 水泥具有直接与新形成的硬组织结合的特性。