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Superoxide production by leukocytes from streptozotocin induced diabetic rats and naturally occurring gingivitis rats.

链脲佐菌素诱导的糖尿病大鼠和自然发生的牙龈炎大鼠的白细胞产生超氧化物。

基本信息

批准号：
62570851
负责人：
MORI Masakazu
金额：
$ 1.22万
依托单位：
Osaka Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570851/
关键词：
Diabetes mellitus Gingivitis Rats Leukocytes 活性酸素

项目摘要

A common oral sign of diabetes mellitus is an increased severity in periodontal disease. In this study, we examined leukocytes abnormalities in streptozotocin diabetes rats. Superoxide (O_2^-) production of polymorphonuclear leukocytes (PMNs) and macrophages (M s) were lower in streptozotocin diabetes rats than in normal rats(p<0.05-0.01). These findings suggest that impared O_2^- production might be one of the factors accounting for impared bactericidal activity of PMNs and M s in diabetics. Additionally, it was investigated the relationship between the severity of gingivitis and abnormal M chemotaxis and O_2^- productionin rats susceptible to naturally occurring gingivitis (Sus rats). Sus rats were examined using plaque index and pocket probing depth. M chemotaxis and O_2^- production in the Sus deep pocket group (over 4.0mm pocket depth) were significantly lower than that in the Sus shallow pocket group (under 3.5mm pocket depth) and normal group (p<0.05 - 0.005). In addition, a negative correlation was found between the severity of gingivitis in Sus rats and M chemotakis and O_2^- production by Opsonized zymosan (p<0.05-0.01). These results suggest that defective M chemotaxis and superoxide production are a pathogenetic factor in destructive periodontal disease in Sus rats.

糖尿病的一个常见口腔症状是牙周病的严重程度增加。在这项研究中，我们研究了链脲佐菌素糖尿病大鼠的白细胞异常。糖尿病大鼠中性粒细胞（PMNs）和巨噬细胞（Ms）超氧阴离子（O_2^-）生成量明显低于正常大鼠（p<0.05-0.01）。提示糖尿病患者PMNs和M s产生的O_2 ~-可能是其杀菌活性增强的原因之一。此外，还研究了自然发生牙龈炎易感大鼠（Sus大鼠）牙龈炎严重程度与M趋化性和O_2^-产生异常的关系。以菌斑指数和牙周袋探诊深度对大鼠进行检测。Sus深囊组（囊袋深度大于4.0mm）的M趋化性和O_2 ~-生成量显著低于Sus浅囊组（囊袋深度小于3.5mm）和正常组（p<0.05 ~ 0.005）。此外，牙龈炎的严重程度与趋化分枝杆菌和酵母多糖产生的O_2 ~-呈负相关（p<0.05-0.01）。这些结果表明，缺陷M趋化性和超氧化物的产生是破坏性牙周病在Sus大鼠的致病因素。