Superoxide production by leukocytes from streptozotocin induced diabetic rats and naturally occurring gingivitis rats.

糖尿病的一个常见口腔症状是牙周病的严重程度增加。在这项研究中，我们研究了链脲佐菌素糖尿病大鼠的白细胞异常。糖尿病大鼠中性粒细胞（PMNs）和巨噬细胞（Ms）超氧阴离子（O_2^-）生成量明显低于正常大鼠（p<0.05-0.01）。提示糖尿病患者PMNs和M s产生的O_2 ~-可能是其杀菌活性增强的原因之一。此外，还研究了自然发生牙龈炎易感大鼠（Sus大鼠）牙龈炎严重程度与M趋化性和O_2^-产生异常的关系。以菌斑指数和牙周袋探诊深度对大鼠进行检测。Sus深囊组（囊袋深度大于4.0mm）的M趋化性和O_2 ~-生成量显著低于Sus浅囊组（囊袋深度小于3.5mm）和正常组（p<0.05 ~ 0.005）。此外，牙龈炎的严重程度与趋化分枝杆菌和酵母多糖产生的O_2 ~-呈负相关（p<0.05-0.01）。这些结果表明，缺陷M趋化性和超氧化物的产生是破坏性牙周病在Sus大鼠的致病因素。

A common oral sign of diabetes mellitus is an increased severity in periodontal disease. In this study, we examined leukocytes abnormalities in streptozotocin diabetes rats. Superoxide (O_2^-) production of polymorphonuclear leukocytes (PMNs) and macrophages (M s) were lower in streptozotocin diabetes rats than in normal rats(p<0.05-0.01). These findings suggest that impared O_2^- production might be one of the factors accounting for impared bactericidal activity of PMNs and M s in diabetics. Additionally, it was investigated the relationship between the severity of gingivitis and abnormal M chemotaxis and O_2^- productionin rats susceptible to naturally occurring gingivitis (Sus rats). Sus rats were examined using plaque index and pocket probing depth. M chemotaxis and O_2^- production in the Sus deep pocket group (over 4.0mm pocket depth) were significantly lower than that in the Sus shallow pocket group (under 3.5mm pocket depth) and normal group (p<0.05 - 0.005). In addition, a negative correlation was found between the severity of gingivitis in Sus rats and M chemotakis and O_2^- production by Opsonized zymosan (p<0.05-0.01). These results suggest that defective M chemotaxis and superoxide production are a pathogenetic factor in destructive periodontal disease in Sus rats.