Superoxide production by leukocytes from streptozotocin induced diabetic rats and naturally occurring gingivitis rats.

糖尿病的一个常见的口腔症状是牙周病的严重程度增加。在这项研究中，我们检测了链脲佐菌素糖尿病大鼠的白细胞异常。链脲佐菌素糖尿病大鼠多形核白细胞(PMN)和巨噬细胞(M S)超氧阴离子自由基(O_2~-)的产生明显低于正常大鼠(P<0.05~0.01)。提示O_2~(2+)产生受损可能是糖尿病患者PMN和M·S杀菌活性降低的原因之一。此外，还探讨了自然牙周炎易感大鼠(SUS大鼠)牙周炎的严重程度与M趋化性异常和O_2~(2+)产生的关系。用斑块指数和口袋探诊深度对SUS大鼠进行检查。SUS深袋组(4.0 mm以上)M趋化性和O_2~-生成量显著低于SUS浅袋组(3.5 mm以下)和正常组(P<0.05~0.005)。此外，SUS大鼠牙周炎的严重程度与趋化支原体和优化酵母多糖产生O_2~(2+)呈负相关(p&lt；0.05-0.01)。这些结果提示M趋化性缺陷和超氧化物产生是SUS大鼠破坏性牙周病的致病因素之一。

A common oral sign of diabetes mellitus is an increased severity in periodontal disease. In this study, we examined leukocytes abnormalities in streptozotocin diabetes rats. Superoxide (O_2^-) production of polymorphonuclear leukocytes (PMNs) and macrophages (M s) were lower in streptozotocin diabetes rats than in normal rats(p<0.05-0.01). These findings suggest that impared O_2^- production might be one of the factors accounting for impared bactericidal activity of PMNs and M s in diabetics. Additionally, it was investigated the relationship between the severity of gingivitis and abnormal M chemotaxis and O_2^- productionin rats susceptible to naturally occurring gingivitis (Sus rats). Sus rats were examined using plaque index and pocket probing depth. M chemotaxis and O_2^- production in the Sus deep pocket group (over 4.0mm pocket depth) were significantly lower than that in the Sus shallow pocket group (under 3.5mm pocket depth) and normal group (p<0.05 - 0.005). In addition, a negative correlation was found between the severity of gingivitis in Sus rats and M chemotakis and O_2^- production by Opsonized zymosan (p<0.05-0.01). These results suggest that defective M chemotaxis and superoxide production are a pathogenetic factor in destructive periodontal disease in Sus rats.