Immunobiological Studies on Root Canal Anaerobic Bacteria in the Mechanism of Development of Periapical Lesion

有强烈的证据表明，根尖周围病变的发生是对持续抗原刺激的免疫病理反应的结果。来自根管系统的厌氧菌可能是引起根尖周组织免疫反应的重要致病因素。因此，本研究的目的是阐明从慢性根尖周病变的根管中分离出的厌氧菌的免疫学潜力。从根管中分离到大量的专性厌氧菌，尤其是类杆菌。研究了厌氧菌细胞组分促进单核细胞迁移、诱导白介素1产生、有丝分裂原和B细胞活化等免疫生物学活性。口腔拟杆菌、口腔杆菌和微小韦隆杆菌等革兰氏阴性杆菌细胞成分的免疫生物学活性强于其他细菌，因此对其进行了I-…研究更多的免疫学潜势是从根管中分离的。结果表明，内毒素和38K蛋白制剂均能诱导免疫生物学活性。用免疫组织化学方法检测了慢性根尖周炎患者根管内免疫致密细胞和根管细菌的分布情况，发现感染根管内的细菌主要为类杆菌、韦氏杆菌、消化链球菌和优生菌，它们最常吞噬在泡沫细胞和/或结缔组织的细胞间隙内，并证实泡沫细胞周围聚集了大量的T、B淋巴细胞。这表明，改变浸润性免疫活性细胞在改变痛经中可能是重要的。提示感染根管内细菌的免疫反应导致根尖周病变的发生，可能是引起根尖周组织免疫反应的潜在抗原。较少

It has been strongly suggested that the periapical lesion develop as a result of immunopathological response to continuous antigenic stimulation. Anaerobic bacteria from root canal systems might be most important pathogenesis capable of inducing immunological reactions in periapical tissue. The purpose of this study, therefore, was clarify the immunological potentials of anaeobic bacteria, was frequently isolated from root canals with chronic periapical lesion. Obligated anaerobes, espcially bacteroides, were isolated in high percentage from root canals. Immunobiological activities of anaerobic bacteria cellular components were investigated on the enhancement of monocytes migration, induction of interleukin 1 production, mitogenicity and oplyclonal B cell activation. Immunobiological activities of cellular components from gram negativebacteria such as Bacteroides buccae, B.oralis and Veillonella paruvula, were stronger than that of other species, and it therefore was investigated the i … More mmunological potentials of B.buccae, was frequently isolatedfrom root canals. It shows that both LPS and 38K protein prepartions from B.buccae induced immunobiological activities. The localization of immunocomptent cells and root canal bacteria in human chronic periapical lesions were examined immunohistochemically, the bacteria predominantly isolated from infected root canals, Bacteroides, Veillonella, Peptostreptococcus and Eubacterium, were found most commonly engulfed materials within the foam cell and/or intercellular space in connective tissue, and it was confirmed that many T,B lymphocytes were accumlated around the foam cells.The presence of high number of t lymphocytes was observed in all of the periapical lesion, but, B lymphocytes and macrophage were significantly more prevalent in periapical granuloma than radicular cyst or apical scar. It suggests that altering the infiltrated immunocompetent cells may be important in changing pathema. These finding indicate that periapical lesion is developed as a result of immunological reactions in bacteria from infected root canals may be potential antigens which induce the immunological reactions in periapical tissue. Less