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Immunobiological Studies on Root Canal Anaerobic Bacteria in the Mechanism of Development of Periapical Lesion

根管厌氧菌在根尖周病变发生机制中的免疫生物学研究

基本信息

批准号：
62570867
负责人：
OSADA Tamotsu
金额：
$ 1.09万
依托单位：
Kanagawa Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570867/
关键词：
Periapical Lesion Root Canal Bacteria Monocyte Migration IL-1 Mitogenicity PBA T lymphocyte B lymphocyte 免疫担当細胞 IL-1 マイトジェン作用 LPS

项目摘要

It has been strongly suggested that the periapical lesion develop as a result of immunopathological response to continuous antigenic stimulation. Anaerobic bacteria from root canal systems might be most important pathogenesis capable of inducing immunological reactions in periapical tissue. The purpose of this study, therefore, was clarify the immunological potentials of anaeobic bacteria, was frequently isolated from root canals with chronic periapical lesion. Obligated anaerobes, espcially bacteroides, were isolated in high percentage from root canals. Immunobiological activities of anaerobic bacteria cellular components were investigated on the enhancement of monocytes migration, induction of interleukin 1 production, mitogenicity and oplyclonal B cell activation. Immunobiological activities of cellular components from gram negativebacteria such as Bacteroides buccae, B.oralis and Veillonella paruvula, were stronger than that of other species, and it therefore was investigated the i … More mmunological potentials of B.buccae, was frequently isolatedfrom root canals. It shows that both LPS and 38K protein prepartions from B.buccae induced immunobiological activities. The localization of immunocomptent cells and root canal bacteria in human chronic periapical lesions were examined immunohistochemically, the bacteria predominantly isolated from infected root canals, Bacteroides, Veillonella, Peptostreptococcus and Eubacterium, were found most commonly engulfed materials within the foam cell and/or intercellular space in connective tissue, and it was confirmed that many T,B lymphocytes were accumlated around the foam cells.The presence of high number of t lymphocytes was observed in all of the periapical lesion, but, B lymphocytes and macrophage were significantly more prevalent in periapical granuloma than radicular cyst or apical scar. It suggests that altering the infiltrated immunocompetent cells may be important in changing pathema. These finding indicate that periapical lesion is developed as a result of immunological reactions in bacteria from infected root canals may be potential antigens which induce the immunological reactions in periapical tissue. Less

它已强烈建议，根尖周病变的发展作为一个结果的免疫病理反应，连续抗原刺激。根管系统中的厌氧菌可能是引起根尖周组织免疫反应的重要致病菌。因此，本研究的目的是阐明慢性根尖周病变根管内常见厌氧菌的免疫学潜能。专性厌氧菌，特别是类杆菌，在高比例的根管分离。研究了厌氧菌细胞组分的免疫生物学活性，包括促进单核细胞迁移、诱导白细胞介素1产生、促有丝分裂和活化前环B细胞。革兰氏阴性菌如颊拟杆菌、口腔B杆菌和小韦荣氏球菌的细胞组分的免疫生物活性较强，因此研究了其免疫生物学活性。 ...更多信息颊B菌的免疫活性常从根管中分离出来。结果表明，从B.buccae中提取的LPS和38 K蛋白均具有免疫生物学活性。对慢性根尖周病患者根管内的免疫细胞和细菌进行了化学定位研究，发现感染根管内主要分离的细菌类杆菌属、韦荣氏球菌属、消化链球菌属和真杆菌属是最常见的吞噬物质，并证实许多T，泡沫细胞周围有B淋巴细胞浸润，所有根尖周病变中均可见大量t淋巴细胞，但根尖肉芽肿中B淋巴细胞和巨噬细胞明显多于根尖囊肿和根尖瘢痕。提示改变浸润的免疫活性细胞可能在改变病理过程中起重要作用。这些结果表明，根尖周病变是由于感染根管内的细菌免疫反应的结果，可能是潜在的抗原，诱导根尖周组织的免疫反应。少