The Study of Establishment on Immunobiological Evaluation of Endodontic Materials and Medicaments

本研究旨在评价7种盖髓材料（Dycal、Life、NU-CAP、Cavitek、EUGEDAIN、Fuji-Iceland Type II和Base cement）对人牙髓成纤维细胞（HPF）的生物学效应，以及6种根管充填材料（CANALS、NU-59、AH-26、Endo-fill、Sealapex、APATITE ROOTSEALER TYPE-II）的生物学效应。细胞生物学效应通过测定IL-1、PGE_2、胶原酶、DNA合成、每种材料溶解后刺激HPF或HPLF的细胞活力和碱性磷酸酶（ALP）活性。盖髓材料获得的结果如下。1）用富士II型离聚物刺激的IL-1 α和IL-1 β的产生显着升高。2）PGE_2）所有供试品刺激HPF均能诱导ALP活性的产生，特别是Dycal刺激后，胶原酶活性增加。3）所有供试品刺激HPF均不能诱导胶原酶活性。4）Dycal、Life和Cavitek刺激HPF后，ALP活性无明显变化。 关于我们 5）所有材料在10倍稀释时都强烈地抑制HPF的DNA合成，然而，发现除了基础水泥和Fuji-Icons II型在10倍稀释时之外，所有材料<-2>几乎没有抑制作用。6<-2>）Fuji-Iceland II型材料对HPF的生长有明显的抑制作用，但在10 ~ 10^0倍稀释度范围内，各材料对HPF的刺激活性不同，实验结果如下：1）APATITE ROOTSEALER II型材料和Endo-fill材料对HPLF刺激后产生IL-1 β的能力不同<-2>，但IL-1 α的产生却不明显; 2）所有供试品刺激HPLF均能诱导PGE_2的产生，尤其是CANALS刺激后PGE_2的产生明显增加; 3）所有供试品刺激HPLF均不能诱导胶原酶的活性; 4）Sealapex刺激HPLF后ALP的活性，5）所有供试品在10^0稀释度下均强烈抑制HPLF的DNA合成，而所有供试品在10_1稀释度下几乎没有抑制作用。6）所有供试品均抑制HPLF的生长<-2>，用每种材料刺激的HPLF的活力取决于10 μ g<-2>和10 μ g稀释度之间的浓度。少

The present study was conducted to assess the biological effects of seven pulp capping materials (Dycal, Life, NU-CAP, Cavitek, EUGEDAIN, Fuji-Ionomer Type II and Base cement) against human pulp fibroblasts (HPF), and six root canal filling materials (CANALS, NU-59, AH-26, Endo-fill, Sealapex, APATITE ROOTSEALER TYPE-II). The cell-biological effects were performed by measuring IL-1, PGE_2, Collagenase, DNA synthesis, Cell viabilities and Alkaline phosphatase (ALP) activity of HPF or HPLF stimulated with dissolution of each material.The results by Pulp Capping Materials obtained were as follows.1) The production of IL-1alpha and IL-1beta stimulated with Fuji-Ionomer Type II were significantly high.2) The PGE_2 production was induced when HPF was stimulated by all test samples, especially increased by stimulation with Dycal.3) The Collagenase activity of HPF was not induced by stimulation with all test samples.4) The ALP activities from HPF stimulated with Dycal, Life, and Cavitek were f … More ound.5) All materials strongly inhibited DNA synthesis of HPF at 10^0 dilution, however all materials except Base cement and Fuji-Ionomer Type-II at 10^<-2> dilution were found to have little inhibition. The inhibition of DNA synthesis by all materials depended on the concentration between 10^<-2> and 10^0 dilution.6) Although Fuji-Ionomer Type II inhibited the growth of HPF distinctly, the viabilities of HPF stimulated with each material depended on the concentration between 10^<-2> and 10^0 dilution.The results by Root Canal Filling Materials obtained were as follows.1) The production of IL-1 beta from HPLF stimulated APATITE ROOTSEALER TYPE II and Endo-fill were determinable, but the production of IL-1 alpha was not determinable at all.2) The PGE_2 production was induced when HPLF was stimulated by all test samples, especially increased by stimulation with CANALS.3) The Collagenase activity of HPLF was not induced by stimulation with all test samples.4) The ALP activities from HPLF stimulated with Sealapex, CANALS and APATITE ROOTSEALER TYPE-II were found.5) All test samples strongly inhibited DNA synthesis of HPLF at 10^0 dilution, however all materials at 10_<-2> dilution were found to have little inhibition.6) All test samples inhibited the growth of HPLF, the viabilities of HPLF stimulated with each materials depended on the concentration between 10^<-2> and 10^0 dilution. Less