Study on binding specificity of anti-gangliosides monoclonal antibodies

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The fine structural specificities of six monoclonal antibodies (MAbs) to ganglioside GD2 were studied. These MAbs were produced by hybridomas obtained from A/J mice immunized with EL4 (C57BL/6 derived-T lymphoma). The binding specificities of these MAbs were found to differ from each other by virtue of their binding to structurally related authentic standard glycosphingolipids as revealed by three different assay systems. The MAbs examined could be divided into three binding types. Three MAbs A1-201, A1- 410, and Al-425 bound specifically to ganglioside GD2 and none of the other gangliosides tested. Two other MAbs Al-245 and Al-267 reacted not only with GD2, but also several other gangliosides. In addition, these MAbs were found to distinguish between different N-acetyl- and N-glycolyl- neuraminic acid derivatives of ganglioside GD3. The last MAb A1-287 reacted with several other gangliosides but with lower avidity than A1-245 and A1-267. These six MAbs, furthermore, reacted with ganglioside GD2 lactones. The reactivities of these antibodies to various ganglioside lactones also differ from each other.