Study on binding specificity of anti-gangliosides monoclonal antibodies

研究了6种抗神经节苷脂GD 2单克隆抗体的精细结构特异性。这些单克隆抗体是通过从用EL 4（C57 BL/6衍生的T淋巴瘤）免疫的A/J小鼠获得的杂交瘤产生的。这些单克隆抗体的结合特异性被发现是不同的，由于它们的结合结构相关的真实的标准鞘糖脂，揭示了三个不同的测定系统。所检测的单克隆抗体可分为三种结合类型。三种MAb A1-201、A1- 410和Al-425特异性结合神经节苷脂GD 2，而不结合测试的其他神经节苷脂。另外两个MAb Al-245和Al-267不仅与GD 2反应，还与其他几种神经节苷脂反应。此外，发现这些单克隆抗体区分神经节苷脂GD 3的不同N-乙酰基-和N-羟乙酰基-神经氨酸衍生物。最后一个MAb A1-287与其他几种神经节苷脂反应，但亲合力低于A1-245和A1-267。此外，这六种单克隆抗体与神经节苷脂GD 2内酯反应。这些抗体对各种神经节苷脂内酯的反应性也彼此不同。

The fine structural specificities of six monoclonal antibodies (MAbs) to ganglioside GD2 were studied. These MAbs were produced by hybridomas obtained from A/J mice immunized with EL4 (C57BL/6 derived-T lymphoma). The binding specificities of these MAbs were found to differ from each other by virtue of their binding to structurally related authentic standard glycosphingolipids as revealed by three different assay systems. The MAbs examined could be divided into three binding types. Three MAbs A1-201, A1- 410, and Al-425 bound specifically to ganglioside GD2 and none of the other gangliosides tested. Two other MAbs Al-245 and Al-267 reacted not only with GD2, but also several other gangliosides. In addition, these MAbs were found to distinguish between different N-acetyl- and N-glycolyl- neuraminic acid derivatives of ganglioside GD3. The last MAb A1-287 reacted with several other gangliosides but with lower avidity than A1-245 and A1-267. These six MAbs, furthermore, reacted with ganglioside GD2 lactones. The reactivities of these antibodies to various ganglioside lactones also differ from each other.