Study on binding specificity of anti-gangliosides monoclonal antibodies

抗神经节苷脂单克隆抗体结合特异性的研究

• 批准号: 62580141
• 负责人: TAI Tadashi
• 金额: $ 1.09万
• 依托单位: Tokyo Metropolitan Institute of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62580141/
• 关键词: Gangliosides; Monoclonal antibody; Tumor antigen; Melanoma; Neuroblastoma; モノクロナ-ル抗体; 細胞障害作用; モノクローナル抗体; メラノーマ; ラクトン

The fine structural specificities of six monoclonal antibodies (MAbs) to ganglioside GD2 were studied. These MAbs were produced by hybridomas obtained from A/J mice immunized with EL4 (C57BL/6 derived-T lymphoma). The binding specificities of these MAbs were found to differ from each other by virtue of their binding to structurally related authentic standard glycosphingolipids as revealed by three different assay systems. The MAbs examined could be divided into three binding types. Three MAbs A1-201, A1- 410, and Al-425 bound specifically to ganglioside GD2 and none of the other gangliosides tested. Two other MAbs Al-245 and Al-267 reacted not only with GD2, but also several other gangliosides. In addition, these MAbs were found to distinguish between different N-acetyl- and N-glycolyl- neuraminic acid derivatives of ganglioside GD3. The last MAb A1-287 reacted with several other gangliosides but with lower avidity than A1-245 and A1-267. These six MAbs, furthermore, reacted with ganglioside GD2 lactones. The reactivities of these antibodies to various ganglioside lactones also differ from each other.

研究了6株抗神经节苷脂GD2的单抗的精细结构特异性。这些单抗是用EL4免疫的A/J小鼠(C57BL/6来源的T淋巴瘤)制备的。通过三种不同的检测系统发现，这些单抗与结构上相关的标准糖鞘糖脂的结合特性是不同的。所研究的单抗可分为三种结合类型。三株单抗A1-201、A1-410和Al-425与神经节苷脂GD2特异性结合，未检测到其他神经节苷脂。另外两个单抗Al-245和Al-267不仅能与GD2反应，还能与其他几种神经节苷脂反应。此外，这些单抗被发现可以区分神经节苷脂GD3的不同N-乙酰和N-乙醇基神经氨酸衍生物。最后一株单抗A1-287与其他几种神经节苷脂反应，但亲和力低于A1-245和A1-267。此外，这6株单抗还能与神经节苷脂GD2内酯反应。这些抗体对不同神经节苷脂内酯的反应性也各不相同。

项目成果

Tadashi Tai.: Biochim.Biophys.Acta. 958. 134-138 (1988)

泰正：生物化学学报。

Tadashi Tai.: J.Biochem.(Tokyo). 103. 313-318 (1988)

Tadashi Tai.：J.Biochem.（东京）。

Tadashi Tai.: "Present Status of Ganglioside in Oncology" Karger Press, (1989)

Tadashi Tai.：“神经节苷脂在肿瘤学中的现状”Karger Press，（1989）

田井 直: Medixal Immunology. 13. 757-762 (1987)

泰闹：医学免疫学 13. 757-762 (1987)

T.Tai: "Different reactivities of monoclonal antibodies to ganglioside lactones." Biochem.Biophys.Acta. 958. 134-138 (1988)

T.Tai：“单克隆抗体对神经节苷脂内酯的不同反应性。”