Studies on immunopathological, epidemiological analysis and control of schistosomiasis japonica in the endemic・area around Dong-ting lake.

环洞庭湖地区日本血吸虫病免疫病理学、流行病学分析及防治研究.

• 批准号: 01044155
• 负责人: KOYAMA Tsutomu
• 金额: $ 2.56万
• 依托单位: National Institute of Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01044155/
• 关键词: Schistosomiasis japonica; Dong-ting lake; Epidemiology; Immunopathology; Hepatic carcinoma; Hepatitis B; Activating T lymphocyte by idiotypic antibodies; Control of intermediate host snail

The newly selected foci were: (A) high prevalent area; (B) low prevalent one; and (C) nearly controlled one. The positive rates of the Schistosoma japonicum egg were 65.4%, 12.2-18.3% and 0% in areas (A), (B) and (C), respectively. In (C), the positive rate of 6.5% was obtained as a result of serological tests (COPT and IHA). Some of the people with the eggs were serologically tested by means of ELISA before and after the treatment with praziquantel. Within six months of the treatment, there occurred a remarkable decrease of the antibody titer against the egg antigen. Three kinds of molluscicides were investigated for their effects in laboratories and fields. Cartap among them showed high activity in field trials. The protozoan parasites of vector snails were also investigated for the purpose of biological control. We investigated heterogeneity of idiotypes of anti-egg antibodies in Chinese patients. The serum samples were tested for activating schistosome-specific T lymphocyte in vitro. The result suggests that there is a regulatory idiotypic antibodies in some patients and that the test seems useful for the analysis of immunopathological mechanisms in schistosomiasis japonica. Pathological features of hepatic carcinoma related to schistosomiasis and hepatitis B were studied on the patients both in China and in Japan.

新选择的疫源地为：（A）高流行区;（B）低流行区;（C）近控制区。虫卵阳性率A区为65.4%，B区为12.2-18.3%，C区为0%。在（C）中，血清学试验（COPT和IHA）的阳性率为6.5%。部分携带虫卵者在吡喹酮治疗前后进行了ELISA血清学检测。在治疗的六个月内，发生了针对卵抗原的抗体滴度的显著降低。对三种灭螺剂进行了室内和现场灭螺效果的研究。其中杀螟丹在田间试验中表现出较高的活性。同时对媒介钉螺的原虫寄生情况进行了调查，以达到生物防治的目的。我们调查了中国患者抗虫卵抗体独特型的异质性。检测血清样品体外激活的溶酶体特异性T淋巴细胞。提示部分患者体内存在调节性独特型抗体，该试验对分析血吸虫病免疫病理机制有一定意义。本文对中日两国血吸虫病和B型肝炎相关性肝癌的病理特点进行了比较研究。

项目成果

Ohta,N.,Edahiro,T.,Ishii,A.,Yasukawa,M.and Hosaka,Y.: "HLA-DQ-controlled T cell response to soluble egg antigen of Schistosoma japonicum in humans." Clinical and Experimental Immunology. 79. (1990)

Ohta,N.、Edahiro,T.、Ishii,A.、Yasukawa,M.和 Hosaka,Y.：“HLA-DQ 控制的 T 细胞对人类日本血吸虫可溶性卵抗原的反应。”

Ohta,N.,Hosaka,Y.,Minai,M.,Hayashi,M.and Miki,Y.: "Disappearance of specific antibodies in patients with chronic schistosomiasis japonica by treatment with praziquantel." Japanese Journal of Medical Science and Biology. 42. 31-38 (1989)

Ohta,N.、Hosaka,Y.、Minai,M.、Hayashi,M. 和 Miki,Y.：“吡喹酮治疗慢性日本血吸虫病患者特异性抗体消失。”

Wong, M., Xia, C., Chen, M., Zhang, C., Mao, P., Nei, G. and Hosaka, Y.: "Laboratory and field assessment of molluscicidal activity of B-2 against Oncomelania hupensis, the vector snail of schistosomiasis in China." Japanese Journal of Medical Science and

Wong, M.、Xia, C.、Chen, M.、Zhang, C.、Mao, P.、Nei, G. 和 Hosaka, Y.：“B-2 对钉螺杀灭软体动物活性的实验室和现场评估

Wong,M.,Xia,C.,Chen,M.,Zhang,C.,Mao,P.,Nei,G.,and Hosaka,Y.: "Laboratory and field assessment of molluscicidal activity of B-2 against Oncomelania hupensis,the vector snail of schistosomiasis in China." Japanese Journal of Medical Science and Biology. 41.

Wong, M.、Xia, C.、Chen, M.、Zhang, C.、Mao, P.、Nei, G. 和 Hosaka, Y.：“B-2 对钉螺杀软体活性的实验室和现场评估

Ohta,N.,Itagaki,T.,Minai,M.,Hirayama,K.and Hosaka,Y.: "Schistosoma japonicum egg antigen-specific T cell lines in man.Induction of helper and suppressor T cell lines and clones in vitro in a patient with chronic schistosomiasis japonica." J.Clinical Inves

Ohta,N.、Itagaki,T.、Minai,M.、Hirayama,K. 和 Hosaka,Y.：“人类日本血吸虫卵抗原特异性 T 细胞系。辅助性和抑制性 T 细胞系和克隆的体外诱导