Physiological Roles of Cell Growthー or Differentiationーfactors and Their Participations in the Pathologic Changes in the Hereditary Abnormal Models.

细胞生长或分化因子的生理作用及其参与遗传异常模型的病理变化。

• 批准号: 01304025
• 负责人: SHIOTA Kunio
• 金额: $ 8.38万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01304025/
• 关键词: SHC rat; anterior pituitary hormones; activin A; transforming growth factorーbeta; cell growth factor; ICGN mice; PC mice; ocd; ocd rat; アクチビン; Fibroblast Growth Factor; マストミス

1) Spontaneously hypercholesterolemic (SHC) rats were originally selected based on their high plasma cholesterol levels from sprague-dawley rats and established as a model for hypercholesterolemia. We have found that SHC rat exhibits abnormal bone metabolism characterized by higher excretion of hydroxyproline and calcium into urine, a high level of serum calcium, or a decrease in bone weight and calcium content in the ash. In SHC rats, the rats exhibits abnormalities in endocrine function including hypersecretions of thyroid stimulating hormone and growth hormone preceding the occurrence of renal dysfunction. These abnormalities seems to depend on the cellular component of hormone producing cells in the anterior pituitary gland.2) Hormone producing cells in the anterior pituitary gland are heterogeneous and are thought to be composed of cells in various stages of differentiation. Since some of cells in adult rats are thought to be stem cells, it is possible that activin A may induce di … More fferentiation of proliferation in such stem cells. The mode of action of activin A on the anterior pituitary gland was investigated using primary cultured cells. In the study, folliclestimulating hormone (FSH) secretory activity was stimulated by activin A in a cell density-dependent manner and that the treatment with activin increased the number of FSH cells. Thus, we found that activin A acts on pituitary cells in the same way as a growth- or differentiation promoting factor. We also demonstrated that the capacity of Activin A to release the 2-cell block and stimulate early embryonal development in vitro.3) Prolactin is known to be a hormone carrying luteotropic action in rats and enhances progesterone secretion. We found that transforming growth factor-beta can suppress luteal 20 alpha-hydroxysteroid dehydrogenase activity and that luteotropic action of PRL is at least in part mediated by TGF-beta.4) Electron microscopic studies of glomerular lesions in hereditary nephrotic mice (ICGN strain) and of epiphyseal cartilage of congenital osteochondrodysplasia rat (ocd/ocd) have been done. Less

1)自发性高胆固醇血症（SHC）大鼠最初基于其来自Sprague-dawley大鼠的高血浆胆固醇水平而被选择，并被建立为高胆固醇血症模型。我们已经发现，SHC大鼠表现出异常的骨代谢，其特征在于尿中羟脯氨酸和钙的排泄量增加，血清钙水平升高，或骨重量和灰分中钙含量降低。在SHC大鼠中，在肾功能障碍发生之前，大鼠表现出内分泌功能的异常，包括促甲状腺激素和生长激素的分泌过多。这些异常似乎取决于垂体前叶中激素产生细胞的细胞成分。2）垂体前叶中激素产生细胞是异质的，被认为是由处于不同分化阶段的细胞组成。由于成年大鼠中的一些细胞被认为是干细胞，因此激活素A可能会诱导干细胞分化。 ...更多信息 在这种干细胞中增殖的分化。使用原代培养的细胞研究激活素A对垂体前叶的作用模式。在这项研究中，激活素A以细胞密度依赖性方式刺激卵泡刺激素（FSH）分泌活性，用激活素处理可增加FSH细胞的数量。因此，我们发现激活素A作为生长或分化促进因子以相同的方式作用于垂体细胞。我们还证明了激活素A在体外释放2-细胞阻滞并刺激早期胚胎发育的能力。3）已知催乳素是一种在大鼠中具有促黄体作用的激素，并增强孕酮分泌。我们发现转化生长因子-β可以抑制黄体20 α-羟基类固醇脱氢酶的活性，并且PRL的促黄体作用至少部分是由TGF-β介导的。4）对遗传性肾病小鼠（ICGN品系）的肾小球病变和先天性骨软骨发育不良大鼠（ocd/ocd）的骺软骨进行了电镜研究。少

项目成果

Noboru Murakami: "Longーterm cultured neurons from rat suprachiasmatic nucleus retain the capacity for circadion oscillation of vasopressin release" Brain Res.(1991)

Noboru Murakami：“长期培养的大鼠视交叉上核神经元保留了加压素释放的昼夜节律振荡能力”Brain Res.（1991）

Tetsuro Katayama: "Effects of activin A on anterior pituitary cells fractionated by centrifugal elusion." Mol. Cell. Endoc.(1991)

Tetsuro Katayama：“激活素 A 对离心洗脱分离的垂体前叶细胞的影响。”

Katusichi Sudo: "Abnormal bone metabolism of spontaneously hyper-cholesterolemic (SHC) rats before and after the onset of renal dysfunction." Endocrinol. Jpn.(1991)

Katusichi Sudo：“自发性高胆固醇血症（SHC）大鼠在肾功能障碍发生前后的骨代谢异常。”

Noboru Murakami: "Long-term cultured neurons from rat suparachiasmatic nucleus retain the capacity for circadian oscillation of vasopressin release." Brain Res.(1991)

Noboru Murakami：“长期培养的大鼠视交叉上核神经元保留了加压素释放的昼夜节律振荡能力。”

Shigetoshi Kurosawa: "A histological study on the skin and hairs of PC (poor coat) mice" Exp.Anim.(1991)

Shigetoshi Kurosawa：“PC（劣质皮毛）小鼠皮肤和毛发的组织学研究”Exp.Anim.(1991)