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Study on regulation of placental functions : Involvement of ovarian factors in the mechanism.

胎盘功能调节研究：卵巢因素参与的机制。

基本信息

批准号：
06454113
负责人：
SHIOTA Kunio
金额：
$ 4.67万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

项目摘要

The placenta plays an essential role in fetal growth and survival. maintenance of maternal metabolic status during pregnancy by virtue of its multiple functions relating to the transport of nutrients and waste between mother and tetus, moduiating of the matemal and fetal immune systems to prevent rejection of each other, and its endocrine function of maintaining the pregnancy. Successful development of errbryo is totally dependent on placental function. Considering these multiple functions, not only functions that are required constantly but also pregnancy stage specific events such as placental lactogen synthesis, the placenta is thought to express an abundance of biologically active molecules. In the series of experiments, we have analyzed and identified the almost 800 proteins which are stage-specifically expressed on the placental plasma membrane of the rat placenta. We have also cloned the cDNAs encoding novel prolactin (PPL) like molecules, PL-lm and PLP-D,in the rat placenta. PL-lm RNA is specitically expressed at mid-pregnancy, and PLP-D is specific to late pregnancy. In addition to these placental PPL-like molecules, we found GD3 and GM3 as the main gangliosides in the placenta. The composition changes stage specific manner in the rat placenta. Using prolonged pregnancy models and the various stage-specific indexes as mentioned above, it became clear that the stage-specific functions of placenta are controlled by matemal ovarian factors. Expression of these stage-specific functions of the placenta is related to the differentiation of trophoblasts and changes in the cellular composition. Furthermore, we got the evidence indicating that methylation of DNA of CpG islands or in the promoter regions is involved in the placental specific gene-expression and differentiation of the placenta.

胎盘在胎儿的生长和存活中起着至关重要的作用。通过其在母亲和胎儿之间运输营养和废物的多种功能，调节母体和胎儿的免疫系统以防止彼此排斥，以及维持妊娠的内分泌功能，维持妊娠期间母体的代谢状态。 errbryo 的成功发育完全取决于胎盘功能。考虑到这些多种功能，不仅是持续需要的功能，而且还有妊娠阶段的特定事件，例如胎盘催乳素合成，胎盘被认为表达了大量的生物活性分子。在这一系列的实验中，我们分析鉴定了近800种在大鼠胎盘胎盘质膜上阶段特异性表达的蛋白质。我们还在大鼠胎盘中克隆了编码新型催乳素 (PPL) 样分子 PL-lm 和 PLP-D 的 cDNA。 PL-lm RNA在妊娠中期特异性表达，PLP-D在妊娠晚期特异性表达。除了这些胎盘 PPL 样分子外，我们还发现 GD3 和 GM3 是胎盘中主要的神经节苷脂。该组合物在大鼠胎盘中以特定方式改变阶段。利用上述延长妊娠模型和各种阶段特异性指标，清楚地表明胎盘的阶段特异性功能受母体卵巢因素控制。胎盘这些阶段特异性功能的表达与滋养层细胞的分化和细胞组成的变化有关。此外，我们得到的证据表明CpG岛或启动子区域的DNA甲基化参与胎盘特异性基因表达和胎盘分化。