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Polymorphism of oligosaccharide structure in glycoconjugaates

复合糖中寡糖结构的多态性

基本信息

批准号：
01304030
负责人：
TAKAHASHI Noriko
金额：
$ 10.24万
依托单位：
Nagoya City University College of Nursing
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

项目摘要

Investigators were classified into 3 groups as follows. Investigators belong to groups 1 and 2 were supported with the information obtained by group 3.Group 1 : Analyses of carbohydrate antigens in cancer cells or germ cells and purification of the monoclonal antibodies.Kannagi R. investigated quantitative and qualitative characterization of cancer associated serum glycoprotein antigens expressing sialosyl- or sialosyl-fucosyl type 1 chain polylactosamin.Yamaguchi T. analyzed antigens on human gastric carcinoma.Imai K. investigated a monoclonal antibody to carbohydrate chain on human hepatocellular carcinoma-associated antigen suppressed tumor growth in nude mice.Tsuji Y. investigated human sperm carbohydrate antigens defined by an antisperm human monoclonal antibody derived from an infertile woman bearing antisperm antibodies in her serum.Group 2 : Glycosylation polymorphism on the cell surface, its structure and function.Muramatsu T. and Ozawa M. investigated polymorphism of carbohydrate moieties of peanut agglutinin receptor isolated from human gastric cancer cells.Hayashi K. investigated oligosaccharide polymorphism of nicotinic acetylcholine receptor from Torpedo californica.Nakashima I. investigated cell type and maturation stage-dependent polymorphism of oligosaccharides on murine lymphocytes and lymphoma cells.Kuroda Y. investigated characterization of tumor associated fucogangliosides from PC-12 phenochromocytoma cells.Group 3 : Development of analytical method for simple, reproducible and sensitive determination of the structures of N-linked oligosaccharides.Takahashi N. investigated two-dimensional mapping technique for separation and characterization of N-linked oligosaccharides.Arata Y. accumulated the data of ^1H NMR measurements for N-linked oligosaccharides.Ishihara H. investigated analytical procedure for determination of sialic acids in glycoproteins.

研究人员被分为以下 3 组。第 1 组和第 2 组的研究人员得到第 3 组获得的信息的支持。第 1 组：分析癌细胞或生殖细胞中的碳水化合物抗原以及纯化单克隆抗体。Kannagi R. 研究了表达唾液酸基或唾液酸基岩藻糖基 1 型链聚乳糖胺的癌症相关血清糖蛋白抗原的定量和定性特征。Yamaguchi T. 分析了人胃癌抗原。Imai K. 研究了针对人肝细胞癌相关抗原碳水化合物链的单克隆抗体抑制了裸鼠中的肿瘤生长。Tsuji Y. 研究了由源自不孕妇女的抗精子人单克隆抗体定义的人精子碳水化合物抗原她血清中的抗精子抗体。第 2 组：细胞表面的糖基化多态性、其结构和功能。Muramatsu T. 和 Ozawa M. 研究了从人胃癌细胞中分离的花生凝集素受体的碳水化合物部分的多态性。Hayashi K. 研究了烟碱乙酰胆碱的寡糖多态性加利福尼亚鱼雷受体。Nakashima I. 研究了小鼠淋巴细胞和淋巴瘤细胞上寡糖的细胞类型和成熟阶段依赖性多态性。Kuroda Y. 研究了来自 PC-12 嗜铬细胞瘤细胞的肿瘤相关岩藻神经节苷脂的特征。第 3 组：开发简单、可重复且灵敏的结构测定分析方法 N-连接寡糖。Takahashi N.研究了用于分离和表征N-连接寡糖的二维作图技术。Arata Y.积累了N-连接寡糖的^1H NMR测量数据。Ishihara H.研究了测定糖蛋白中唾液酸的分析程序。