Comprehensive Study on the Effective Use of Laboratory Animals which have been Developed and held in Different Institutions

不同机构开展并举办的实验动物有效利用综合研究

• 批准号: 01304055
• 负责人: HAYAKAWA Jun-ichiro
• 金额: $ 6.91万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01304055/
• 关键词: Laboratory animals; Information; Database; Microbiology; Genetics; Embryo freezing; Embryo transportation; 疾患モデル; デ-タ・ベ-ス

The results of this two-year co-operative research indicate that at the present the following methods on information, quality control, transportation of laboratory animals are best for the mutual utilization of the animals between the different institutions.(1) Construction and use of database. The distribution of diskette which is operated by MS-DOS is the most effective to use the database on the laboratory animals, because most institutions have installed microcomputers which operated by the system but not on-line operated computer system.(2) Quality control of animals. ELISA kit for the detection of the antibodies against the specific pathogens is simple and effective for microbiological quality control. Enzyme electrophoresis by a simple device which developed during this research could be used for genetic quality control of mice and rats.(3) Transportation of animals. Non-frozen and frozen embryos can be transferred between the institutions. The methods are promised future use for the transportation of animals.

经过两年的合作研究，认为目前在实验动物的信息、质量控制、运输等方面采取以下几种方法是最有利于实验动物在不同机构间的相互利用。(1)数据库的建设和使用。由于大多数机构都安装了由MS-DOS操作的微型计算机，而不是在线操作的计算机系统，因此分发由MS-DOS操作的软盘是使用实验动物数据库最有效的方法。(2)动物的质量控制。ELISA试剂盒检测特异性病原体抗体简便、有效，可用于微生物学质量控制。本研究开发的酶电泳装置简单，可用于大、小鼠遗传质量控制。(3)动物的运输。非冷冻和冷冻胚胎可以在机构之间转移。这些方法有望在未来用于运输动物。

项目成果

S. Tanaka, K. Moriwaki et al.: "Gene responsible for deficient activity of the subunit of C8, eighth component of complement is located on chromosome 4." Immunogenetics. 33. 18-23 (1991)

S. Tanaka、K. Moriwaki 等人：“补体第八个组成部分 C8 亚基活性缺陷的基因位于 4 号染色体上。”

T.Koizumi,M.Katsuki,M.Kimura,J.Hayakawa: "Localization of the gene encoding myelin basic protein in mouse chromosome 18E3→4 and rat chromosome 1p11→p12." Cytogenetics & Cell Genetics.

T. Koizumi、M. Katsuki、M. Kimura、J. Hayakawa：“编码髓磷脂碱性蛋白的基因在小鼠染色体 18E3→4 和大鼠染色体 1p11→p12 中的定位。”

K.Koizumi,J.Hayakawa,H.Nikaido: "Blood ammonia concentration in mice:normal reference values and changes during growth." Laboratory Animal Science. 40. (1990)

K.Koizumi，J.Hayakawa，H.Nikaido：“小鼠血氨浓度：正常参考值和生长过程中的变化。”

T. Higashiguchi, J. Yamada et al.: "Identification of inbred strains of rats by DNA fingerprints using chemiluminescence." Transpl. Proc.22. 2564-2565 (1990)

T. Higashiguchi、J. Yamada 等人：“利用化学发光通过 DNA 指纹鉴定大鼠近交系。”

T.Sagai,F.Bonhomme,K.Moriwaki,et al.: "New evidence for trans-species evolution of the H-2 class I polymorphism." Immunogenetics. 30. 89-98 (1989)

T.Sagai、F.Bonhomme、K.Moriwaki 等人：“H-2 I 类多态性跨物种进化的新证据。”