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Comparative Study of Allograft Rejection in Man and Rat : Analysis of Activated Monocytes Involing in Acute Rejection.

人和大鼠同种异体移植排斥的比较研究：参与急性排斥的活化单核细胞分析。

基本信息

批准号：
01440067
负责人：
SONODA Takao
金额：
$ 7.17万
依托单位：
Osaka University Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

Purpose : To find a new immunological indicator for allograft rejection in animal model of experimental transplantation, we investigated whether a SPFC (spontaneous plaque-forming cell) as activated monocytes was involved in allograft rejection and whether adhesion molecules of complement receptor 3 (CR3) was involved in hemolysis of SPFC.Methods and Results : (1) To evaluate SPFC response in skin allograft transplantation of rats, two different combinations of rats disparate with a H-1 histocompatibility locus was chosen. SPFC effector was identified in the infiltrating cells of rejecting transplanted grafts. Numbers of SPFC-effector at peak response of each group in the two experimental transplantation was significantly different. Since SPFC is regulated by a subsets of T-lymphocytes in man, the present findings suggested that SPFC in rats also may be regulated by T-lymphocytes and reflected a histoincompatibility. (2) The Mo-1, anti-human CR3 monoclonal antibody suppressed in vitro hemolysis of SPFC in man by adding the SPFC assay. The OX42, anti-rat CR3 monoclonal antibody also inhibited in vitro hemolysis of SPFC in rats. The CR3 molecule involves in hemolysis of SPFC in both man and rat.Conclusions : The SPFC as cellular effector of activated monocytes may be a useful immunologic indicator for manipulating allograft rejection.

目的：为了寻找一种新的免疫学指标，在实验性移植动物模型中，我们研究了SPFC是否方法：采用免疫组化方法检测SPFC溶血反应中补体受体3（CR 3）粘附分子是否参与了SPFC的溶血反应。（1）为评价SPFC在大鼠同种异体皮肤移植中的反应，选择了两种不同的具有H-1组织相容性位点的大鼠组合。SPFC效应物在排斥移植物的浸润细胞中被鉴定。两种实验移植中各组峰值反应时的SPFC效应细胞数差异有统计学意义。由于人的SPFC受T淋巴细胞亚群的调节，本研究结果表明，大鼠的SPFC也可能受T淋巴细胞的调节，并反映了组织不相容性。(2)Mo-1，抗人CR 3单克隆抗体通过添加SPFC测定抑制人中SPFC的体外溶血。抗大鼠CR 3单克隆抗体OX 42也能抑制SPFC的体外溶血作用。CR 3分子参与了人和大鼠SPFC的溶血反应。结论：SPFC作为活化单核细胞的效应细胞，可能是一种有效的调控同种异体排斥反应的免疫学指标。