Graft rejection mechanism by monocytes and specific immunosuppression.

单核细胞和特异性免疫抑制的移植排斥机制。

• 批准号: 60480357
• 负责人: SONODA Takao
• 金额: $ 3.97万
• 依托单位: Osaka University Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480357/
• 关键词: Renal Transplantation; Monocytes; Rejection; インフルエンザウイルス

We investigated whether a novel monocyte, or spontaneous plaque-forming cell (SPFC), is related with renal allograft rejection in man , and how SPFC generation may be regulated in vitro. A auto-rosette-forming T cell (ARFC-T) may act as helper cell of SPFC-monocyte, while non-ARFC-T and ARFC-monocyte may act as suppressor of SPFC generation. Other environmental antigens, such as influenza virus, cytomegalovirus and E.Coli lipopolyssacharide, and allo-PBMC of man can affect the SPFC generation. In vivo SPFC response can be reflected in the invading cells from rejected grafts, detected by the in vitro MLC-SPFC assay. A definite correlation between graft survival and in vitro SPFC response was observed, that is, an excellent renal allograft survival was shown in the non-response patients of SPFC. The present results suggest that a novel monocyte, SPFC, may relate to renal allograft rejection and that a further study of SPFC-monocyte will give a key to open a new immunosuppression.

我们研究了一种新型单核细胞或自发斑块形成细胞 (SPFC) 是否与人类肾同种异体移植排斥相关，以及如何在体外调节 SPFC 的生成。自花环形成 T 细胞 (ARFC-T) 可能充当 SPFC 单核细胞的辅助细胞，而非 ARFC-T 和 ARFC-单核细胞可能充当 SPFC 生成的抑制细胞。其他环境抗原，例如流感病毒、巨细胞病毒和大肠杆菌脂多糖以及人的同种异体PBMC也可以影响SPFC的产生。体内 SPFC 反应可以反映在来自排斥移植物的入侵细胞中，通过体外 MLC-SPFC 测定进行检测。观察到移植物存活率与体外SPFC反应之间存在明确的相关性，即在SPFC无反应的患者中显示出极好的同种异体肾移植物存活率。目前的结果表明，一种新型单核细胞SPFC可能与移植肾排斥反应有关，对SPFC单核细胞的进一步研究将为开启新的免疫抑制提供钥匙。

项目成果

Michio Ishibashi, Yasuji Ichikawa, Minato Takaha and Takao Sonoda.: "Monocyte-mediated hemolytic response in renal transplant patients." Transplant Proc.17. 2644-2647 (1985)

Michio Ishibashi、Yasuji Ichikawa、Minato Takaha 和 Takao Sonoda：“肾移植患者中单核细胞介导的溶血反应。”

Michio Ishiabshi, Yukito Kokado, Shiro Takahara, Yasuji Ichikawa and Takao Sonoda.: "Cellular immnue response against human ed cell antigens and renal allograft rejection." Transplant Proc. (1987)

Michio Ishiabshi、Yukito Kokado、Shiro Takahara、Yasuji Ichikawa 和 Takao Sonoda：“针对人类 ed 细胞抗原的细胞免疫反应和肾同种异体移植排斥。”