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Studies on the potential hazards of trace amounts of dental materials dissolution

微量牙科材料溶解的潜在危害研究

基本信息

批准号：
01440078
负责人：
SATO Atsushige
金额：
$ 10.11万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

Dental materials are dissolved in the body fluid into trace amounts of components, which will make primary factors to bring side effects. Thus, it is important to clarify the dissolution kinetics and the toxicity of dissolved components. The metal elements in dissolution were quantitated by ICP and atomic absorption spectrophotometry. The potential hazards, particularly allergenicity and mutagenicity, of dental materials were investigated from the dose-response relationship on the dissolved products.1. Dissolution of titanium alloyThe total amounts of titanium dissolved from 100 mg of powdered alloys, Ti6Al4V and Ti5A12.5Fe, were 1045 and 1016 ng, respectively during 14 weeks immersion in human saliva. The total amounts of aluminum dissolved from 100 mg of the alloys were 165 and 480 ng, respectively during 6 weeks immersion. The continuous trace amounts of dissolution was observed from each alloy, suggesting the possibility of genotoxicity and allergenicity.2. Allergenicity of nickel and cadmiumThe quantitation of the blood nickel and allergenicity was compared between mice of NiCl_2-water breeding and mice of subcutaneous nickel implantation.1) The anti-nucleus antibody (ANA) was not induced by oral administration of nickel into ICR of BALB/c mice. The ANA was induced by oral administration of cadmium with a frequency of 17% of ICR mice.2) The lymphocyte blast formation by LPS, Con-A and PHA was enhanced by oral administration of NiCl_2 into BALB/c mice. The blast formation by Con-A was increased by subcutaneous implantation of NiCl_2.3) The relative frequency of mice spleen T cell versus B cell measured by flowcytometry was not different between the control and the nickel- or cadmium-administered mice.4) The frequency of suppressor T cell and helper T cell was significantly decreased in the ICR mice of subcutaneous cadmium-implantation, as compared to the control.

牙科材料在体液中溶解成微量成分，这将成为带来副作用的首要因素。因此，澄清溶解动力学和溶解组分的毒性非常重要。采用ICP和原子吸收分光光度法对溶出液中的金属元素进行定量。从溶解产物的剂量-反应关系研究牙科材料的潜在危害，特别是致敏性和致突变性.钛合金的溶解在14周的人唾液中，从100 mg粉末合金Ti 6Al 4V和Ti 5Al 12.5Fe中溶解的钛的总量分别为1045和1016 ng。在6周的浸泡期间，从100 mg合金中溶解的铝的总量分别为165和480 ng。从每种合金中观察到连续的微量溶解，提示可能存在遗传毒性和过敏性.镍、镉的致敏性比较了NiCl_2-水饲养小鼠和皮下注射镍的小鼠的血镍含量和致敏性。经口给予NiCl_2可增强LPS、Con-A和PHA诱导的淋巴母细胞形成。3）流式细胞仪检测小鼠脾T细胞和B细胞的相对频率在对照组和镉、镍皮下埋植组间无显著性差异。4）与对照组相比，镉皮下埋植组小鼠抑制性T细胞和辅助性T细胞的相对频率均显著降低。