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Studies on the biological response of fumes and airborne particulates from dental materials during their routine use.

研究牙科材料在日常使用过程中产生的烟雾和空气中的颗粒物的生物反应。

基本信息

批准号：
03454446
负责人：
SATO Atsushige
金额：
$ 4.16万
依托单位：
TOKYO MEDICAL and DENTAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

项目摘要

The purpose of this study was to investigate the effect of particlaties from vaporizing in casting or polishing of dental materials on the morphology and viability of murine macrophages. The mice peritoneal macrophages were cultured in Eagle's MEM supplemented with 10% fetal calf serum and incubated for 24 hrs later with Ni-Cr alloy particles. Cell viability of the macrophages was significantly decreased following 24hr exposure of Ni-Cr alloy particles. After phagocytosis more cell death were observed when the Ni-Cr alloy with Be was compared to the Ni-Cr alloy without Be. We also studied the effect of PMMA particles on cell morphology, viability and superoxide release of rat peritoneal macrophages. Rat macrophages in RPMI 1640 supplemented with 20% fetal bovine serum were exposed to 200, 500 and 1500 mug/ml particles for 90 min. For the morphological experiment the macrophages were fixed with 2.5% glutaraldephyde in 0.1 M phosphate pH 7.4 buffer and processed for transmission or scanning electrom microscopy. For the superoxide release experiment, macrophages were incubated in the buffer solution for 90 min at 37ﾟC.The amount of superoxide released was determined by the reduced cytochrome assay. Studies with SEM and TEM indicated that the macrophages phagocytosed PMMA or Ostron particles. Superoxide release from the macrophages was increased from 157.6(〕SY.+-.〔)3.1 to 212.5(〕SY.+-.〔)12.2n mole/mg protein after 90 min of exposure to the PMMA particles. Ostron particles also induced the O_2^- release from the macrophages. In conclusion, it can be stated that particles generated in dental clinics may be caused cell damage and cell death due to phagocytosis of large amount of particles.

本研究的目的是研究牙科材料铸造或抛光过程中蒸发的颗粒对小鼠巨噬细胞形态和活力的影响。将小鼠腹腔巨噬细胞培养于添加10%胎牛血清的Eagle’s MEM中，并与Ni-Cr合金颗粒孵育24 h。Ni-Cr合金颗粒暴露24小时后，巨噬细胞活力明显降低。与不含Be的Ni-Cr合金相比，含Be的Ni-Cr合金在吞噬后的细胞死亡更多。我们还研究了PMMA颗粒对大鼠腹腔巨噬细胞形态、活力和超氧化物释放的影响。将RPMI 1640中添加20%胎牛血清的大鼠巨噬细胞分别暴露于200、500和1500马克杯/毫升颗粒中90分钟。形态学实验中，巨噬细胞用2.5%戊二醛固定在0.1 M磷酸pH 7.4缓冲液中，用透射电镜或扫描电镜观察。超氧化物释放实验：巨噬细胞在37℃的缓冲液中孵育90 min。通过还原细胞色素法测定超氧化物的释放量。扫描电镜和透射电镜研究表明，巨噬细胞吞噬PMMA或介子颗粒。暴露于PMMA颗粒90 min后，巨噬细胞的超氧化物释放量从157.6(]SY.+-.[)3.1增加到212.5(]SY.+-. [)12.2n mol /mg蛋白。Ostron粒子也能诱导巨噬细胞释放O_2^-。综上所述，牙科诊所中产生的颗粒可能由于大量颗粒被吞噬而造成细胞损伤和细胞死亡。