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Inhalation anesthetics and oncogene

吸入麻醉剂和癌基因

基本信息

批准号：
01440063
负责人：
SHIMADA Yasuhiro
金额：
$ 10.43万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01440063/
关键词：
Oncogene c-myc N-myc Inhalation anesthetics HLA-DR ICAM-1 cーmyc Nーmyc HLAーDR ICAMー1 Cーmyc HLAーDR抗原がん遺伝子蛋白 cーmyc遺伝子 cーmyc蛋白

项目摘要

Although almost cancer patients are undergone with general anesthesia, a few researchers and anesthesiologists are interest in the interaction between cancer cells and anesthesia. So we have been doing researches of the influence of anesthesia on cancer cells in order to try to establish the best anesthesia for cancer patients. We put on a focus on "oncogene". We guessed that anesthesia agents might influence oncogene.We studied as follows; (1)halothane and the expression of c-myc protein in human leukemia cells in vitro, (2)halothane and the amplification of N-myc in neuroblastoma in vitro. Results were as follows; (1)the expression of c-myc protein did not change during/after halothane exposure, (2)the amplification of N-myc did not also change during/after halothane exposure.As far as our results, anesthesia did not influence oncogene. There are many other oncogenes. Some of other oncogene might change during/after anesthesia.Furthermore, we did other researches. We tried to evaluate the change of antigens in cancer cells during/after anesthesia in vitro. We studied as follows; (1)halothane and the expression of HLA-DR antigen in melanoma cells, (2)halothane/sevoflurane and the expression of intercellular adhesion molecule-1 (ICAM-1) in melanoma cells. The results were as follows; (1)the expression of HLA-DR in melanoma cells increased after halothane exposure, (2)the expression of ICAM-1 in melanoma cells decreased during halothane/sevoflurane exposure.These results suggested that anesthetic agents influence on antigen expression in cancer cells, and that the change might decrease the recognition of immune surveillance. Anesthetic agents might deteriorate cancer patients.Anesthesiologists and surgeons should be aware that anesthesia might deteriorate cancer patients, and had better continue to do research on the relation between cancer and anesthesia.

尽管大多数癌症患者都接受全身麻醉，但一些研究人员和麻醉师对癌细胞与麻醉之间的相互作用感兴趣。因此，我们一直在研究麻醉对癌细胞的影响，试图为癌症患者建立最佳的麻醉方法。我们把重点放在“致癌基因”上。本实验研究了氟烷对体外培养的人白血病细胞c-myc蛋白表达的影响，氟烷对体外培养的神经母细胞瘤细胞N-myc扩增的影响。结果如下：（1）氟烷暴露前后c-myc蛋白表达无明显变化，（2）氟烷暴露前后N-myc扩增无明显变化，麻醉对癌基因表达无影响。还有许多其他的致癌基因。其他一些癌基因在麻醉过程中或麻醉后也可能发生变化。我们尝试在体外研究麻醉过程中和麻醉后癌细胞抗原的变化。我们研究如下：（1）氟烷与黑色素瘤细胞HLA-DR抗原的表达;（2）氟烷/七氟醚与黑色素瘤细胞细胞间粘附分子-1（ICAM-1）的表达。结果如下：（1）氟烷暴露后黑色素瘤细胞HLA-DR表达增加，（2）氟烷/七氟醚暴露后黑色素瘤细胞ICAM-1表达减少，提示麻醉剂影响癌细胞抗原表达，可能降低了免疫监视的识别能力。麻醉剂可能使癌症病人恶化，麻醉医生和外科医生应该意识到麻醉可能使癌症病人恶化，最好继续研究癌症与麻醉的关系。