Isolation and analysis of yeast genes involved in meiotic first division

酵母减数分裂第一次分裂相关基因的分离与分析

• 批准号: 63540539
• 负责人: SHIMODA Chikashi
• 金额: $ 1.15万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63540539/
• 关键词: Meiosis; Fission yeast; Gene cloning; Transcription; Cold-sensitive mutant; 低温感受性突然変異; 遺伝子クローニング

We have studied the genes responsible for the meiotic first division in the fission yeast Schizosaccharomyces pombe.1. Isolation and genetic analysis of the mutant J13 The cold-sensitive mutant which was arrested at the prophase of meiosis I was obtained from an S. pombe homothallic strain by ethylmethane sulfonate mutagenesis. The mutant, named J13, was unable to complete meiosis I at 18ﾟC, but formed normal 4-spored asci at 30ﾟC. Genetic analyses revealed that J13 harbored a single recessive mutation and defined a new genetic locus, mei10. The mei10 gene was mapped in the vicinity of the centromere on chromosome II.2. Structure and expression of the mei4 gene The 2.8kb DNA fragment containing the mei4 gene was cloned and its nucleotide sequence was determined. The open reading frame composed of 356 codons was found. This predicted mei4 gene product of 39 kDa was supposed to be hydrophilic and rich in serine residues. There were no known proteins which resembled the mei4 protein in the amino acid sequence. Northern analysis showed that the mei4 gene was preferentially transcribed under nitrogen- free medium. This transcriptional activation of mei4 required neither the heterozygosity at the mating-type locus nor the mei2 gene function which were known to be prerequisite to the initiation of meiosis.

我们研究了裂殖酵母裂殖酵母中负责减数分裂第一次分裂的基因。突变体J13的分离和遗传分析从一株S.通过甲基磺酸乙酯诱变获得粟酒裂殖酵母同宗配合物菌株。该突变体命名为J13，在18 ℃不能完成减数分裂I，但在30 ℃形成正常的4孢子子囊。遗传分析表明，J13含有一个隐性突变，并定义了一个新的遗传位点mei10。mei10基因被定位在染色体II.2上的着丝粒附近。mei4基因的结构与表达克隆了含有mei4基因的2.8kb DNA片段，并测定了其核苷酸序列。结果表明，mei4基因的开放阅读框由356个密码子组成，推测其分子量为39 kDa，具有亲水性，富含丝氨酸残基。没有已知的蛋白质类似的mei4蛋白质的氨基酸序列。北方分析表明，mei 4基因在无氮培养基下优先转录。mei4的转录激活既不需要交配型位点的杂合性，也不需要mei2基因的功能，而这是减数分裂起始的先决条件。

项目成果

M.Kishida: "A cold-sensitive spo14 mutation affecting ascospore formation in the fission yeast Schizosaccharomyces pombe." Plant & Cell Physiol.31(1990). 31. (1990)

M.Kishida：“冷敏感 spo14 突变影响裂殖酵母裂殖酵母中子囊孢子的形成。”

Kishida, M., A. Hirata and C. Shimoda: "A cold-sensitive spol4 mutation affecting ascospore formation in the fission yeast Schizosaccharomyces pombe." Plant Cell Physiol. 31 (1990).

Kishida, M.、A. Hirata 和 C. Shimoda：“冷敏感 spol4 突变影响裂殖酵母裂殖酵母中子囊孢子的形成。”

Nishiwaki, S., K. Tanaka, H. Fujioka, T. Aono and C. Shimoda: "Expression of the S. pombe mei4 gene responsible for the meiotic first division."

Nishiwaki, S.、K. Tanaka、H. Fujioka、T. Aono 和 C. Shimoda：“负责减数分裂第一次分裂的粟酒裂殖酵母 mei4 基因的表达。”

H.Fujioka: "A mating-type-specific sterility gene map1 is required for transcription of a mating-type gene mat1-Pi in the fission yeast Schizosaccharomyces pombe." FEMS Microbiol.Lett.60:45-48(1989). 60. 45-48 (1989)

H.Fujioka：“裂殖酵母裂殖酵母中交配型基因 mat1-Pi 的转录需要交配型特异性不育基因 map1。”

H.Fujioka: FEMS Microbiol.Lett.

H.Fujioka：FEMS Microbiol.Lett。