Molecular arrangement of NADP-photoreducing system in chloroplasts.

叶绿体中 NADP 光还原系统的分子排列。

• 批准号: 63540532
• 负责人: SHIN Masateru
• 金额: $ 1.22万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63540532/
• 关键词: chloroplast; ferredoxin-NADP reductase; FNR-L; FNR-S; NADP photoreducing activity; photosystem I particle; Thylakoids; フェレドキシンーNADP還元酵素; 分子構築

Two molecular forms of ferredoxin-NADP reductase (FNR), small from (FNR-S) and large form (FNR-L), have been known to exist in chloroplasts. When spinach chloroplasts were incubated in a dilute Tris-HCl buffer at neutral pH, almost all FNR-L was easily released from thylakoids within 1 hour. Rebinding of FNR-L to the FNR-L-removed thylakoids markedly restored its NADP photoreducing activity. Restoration of the activity by rebinding of FNR-S was considerably small, suggesting that FNR-S does not participate in the NADP photoreducing reaction on the surface of thylakoids. It was concluded that FNR-L was located on the surface of thylakoids to constitute an NADP reductase complex to reduce NADP in the non-cyclic electron flow.FNR-S remained in thylakoids after extraction of FNR-L, suggesting that this FNR is located in some internal part of thylakoids. It was found to be associated with photosystem I particles. It seems that FNR-S functions as a ferredoxin-plastoquinone reductase in the cyclic electron flow, but not as an NADP reductase in the non-cyclic electron flow.

铁氧还蛋白-NADP还原酶（FNR）的两种分子形式，即小形式（FNR-S）和大形式（FNR-L），已知存在于叶绿体中。当菠菜叶绿体在中性pH的Tris-HCl缓冲液中孵育时，几乎所有的FNR-L都容易在1小时内从类囊体中释放出来。FNR-L与去除FNR-L的类囊体的再结合显著恢复了其NADP光还原活性。通过FNR-S的重新结合的活性的恢复是相当小的，这表明FNR-S不参与NADP光还原反应的类囊体表面上。FNR-L位于类囊体表面，与NADP还原酶形成复合物，在非循环电子流中还原NADP，而FNR-S在提取FNR-L后仍保留在类囊体中，表明FNR位于类囊体内部。它被发现与光系统I粒子有关。FNR-S似乎在循环电子流中起铁氧还蛋白-质体醌还原酶的作用，但在非循环电子流中不起NADP还原酶的作用。

项目成果

M.Shin: "Structure and function of ferredoxin-NADP reductase complex." Proceedings of the VIIIth International Congress on Photosynthesis. (1990)

M.Shin：“铁氧还蛋白-NADP 还原酶复合物的结构和功能。”

M.Shin,M.Tsujita,H.Tomizawa,N.Sakihama,K.Kamei,R.Oshino: "Proteolytic degradation of ferredoxin-NADP reductase during purification from spinach." Archives of Biochemistry and Biophysics. (1990)

M.Shin、M.Tsujita、H.Tomizawa、N.Sakihama、K.Kamei、R.Oshino：“菠菜纯化过程中铁氧还蛋白-NADP 还原酶的蛋白水解降解。”

M. Shin, M. Tsujita, H. Tomizawa, N. Sakihama K. Kamei, R. Oshino: "Proteolytic degradation of ferredoxin-NADP redctase during purification from spinach." Archives of Biochemistry and Biophysics. (1990)

M. Shin、M. Tsujita、H. Tomizawa、N. Sakihama K. Kamei、R. Oshino：“菠菜纯化过程中铁氧还蛋白-NADP 还原酶的蛋白水解降解。”

M. Shin: "Structure and function of ferredoxin-NADP redctase complex." Proceedings of VIIIth International Congress on Photosynthesis 1990.

M. Shin：“铁氧还蛋白-NADP 还原酶复合物的结构和功能。”

M.Shin,M.Tsujita,H.Tomizawa,N.Sakihama,K.Kamei,R.Oshino: "Proteolytic degradation of ferredoxin-HADP reductase during purification from spinach." Archives of Biochemistry and Biophysics. (1990)

M.Shin、M.Tsujita、H.Tomizawa、N.Sakihama、K.Kamei、R.Oshino：“菠菜纯化过程中铁氧还蛋白-HADP 还原酶的蛋白水解降解。”