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Role of Lower Brain Stem in Regulating Sexual Behaviors in Male and Female Rats.

下脑干在调节雄性和雌性大鼠性行为中的作用。

基本信息

批准号：
63540600
负责人：
YAMANOUCHI Korehito
金额：
$ 1.34万
依托单位：
Waseda University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63540600/
关键词：
sexual behaviors lordosis mounting male and female rats raphe nuclei medulla oblongata brain lesions serotonin ロ-ズ-シス雄性行動テストステロン雄ラットロードーシスエストロゲンラット

项目摘要

Roles of the medullary raphe nuclei in regulating female and male sexual behaviors were investigated in rats.In ovariectomized rats, the raphe obscurus nucleus (ROBN) or the raphe magnus nucleus (RMGN) was destroyed by radio frequency lesion generator. Four-5 weeks after operations, all females were implanted with a Silastic tubing containing estradiol (E_2). Sexual behavioral tests were started 2 days after E_<> implantations and carried out daily for 5 days. ROBN lesion-females showed high levels of lordosis quotient (LQ) in the first test, when compared with those of non-brain surgery and sham operated females. The mean LQ of RMGN lesion group was comparable to those of control groups. In addition, incidence of ear-wiggling in ROBN lesion group was higher than those in other groups. These results suggest that the raphe obscurus nucleus play an inhibitory role in regulating female rat sexual behaviors.In castrated male rats, ROBN or RMGN was destroyed. Four-5 weeks after operation, all males were implanted Silastic tubings containing testosterone. Sexual behavior tests were started 1 week after testosterone administration and 3 tests were carried out 1 week intervals. In ROBN lesion group, frequencies of mounting, intromission and ejaculation were lower than those in RMBN lesion, non-brain surgery and sham operated groups. These results suggest that in contrast to the female case, the raphe obscurus nucleus play a facilitatory role in regulating male rat sexual behaviors. Furthermore, after treatment with p-chlorophenylalanine (PCPA, an inhibitor for serotonin synthesis), ROBN lesion-males showed higher frequencies of sexual behaviors than those in non-PCPA treated ROBN lesion males, but the frequencies were lower than those in PCPA treated non-brain surgery control males. Thus, PCPA may act on serotonergic neurons other than those in the ROBN and facilitate male sexual behaviors in male rats.

用射频毁损器损毁去卵巢大鼠中缝隐核（ROBN）或中缝大核（RMGN），观察中缝髓核在调节雌性和雄性性行为中的作用。术后4 ~ 5周，植入含雌二醇（E_2）的硅胶管。性行为测试在E_<<>刺激后2天开始，每天进行，持续5天。ROBN病变雌性动物在第一次测试中显示出高水平的脊柱前凸商（LQ），与非脑部手术和假手术雌性动物相比。RMGN病变组LQ均值与对照组相似。此外，ROBN病变组的耳摆动发生率高于其他各组。这些结果提示中缝隐核对雌性大鼠的性行为具有抑制作用，去势雄性大鼠的ROBN或RMGN被破坏。术后4 -5周，所有雄性动物均植入含睾酮的硅橡胶管。在睾酮给药后1周开始性行为测试，间隔1周进行3次测试。ROBN毁损组的爬、插入和射精频率低于RMBN毁损组、非脑手术组和假手术组。这些结果表明，与雌性大鼠相比，中缝隐核在调节雄性大鼠性行为中起易化作用。此外，治疗后与p-氯苯丙氨酸（PCPA，5-羟色胺合成的抑制剂），ROBN病变男性表现出较高的频率比那些在非PCPA治疗ROBN病变男性的性行为，但频率低于那些在PCPA治疗的非脑手术对照男性。因此，PCPA可能作用于ROBN以外的多巴胺能神经元，促进雄性大鼠的雄性性行为。