Neural regulatory systems for sexual and maternal behaviors and the sexual differentiatio

性行为和母性行为以及性别分化的神经调节系统

• 批准号: 14540618
• 负责人: YAMANOUCHI Korehito
• 金额: $ 2.24万
• 依托单位: Waseda University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14540618/
• 关键词: lordosis; sexual differentiation; septum; estrogen beta receptor; serotonin; estrogen; maternal behaior; neonatal period; 性行動; セロトニン神経; 5-HT1A受容体; 新生期エストロゲン; 性差; GABA受容体; 脳の性分化; 中脳中心灰白質; 5HT1A受容体; ゲニステイン; ラット; 神経とレーサー; ペプチド神経伝達物質

1.lordosis-inhibitiory influence in the lateral systemThe retrograde tracer (Fluoro-Gold, FG) was injected into the midbrain central gray(MCG) and immunohistochemical analysis for FG, estrogen a and βreceptors in the lateral septum(LS) was performed in male and female rats. As the results, (1)Number of FG-positive cells in the LS in females was larger than that in males, (2)Numerous βreceptor-immunopositive cells were found in the LS, compared to the a receptor immunopositive cells. Number of these cells containing estrogen β and α receptors was not influenced by exogenous estrogen. (3)Rate of β receptor containing cells in FG positive neurons in the LS was less than 20% in both male and female rats. When estradiol benzoate(EB) was injected to female rats on day 5 after birth and immunohistochemical analysis was performed after injection of FG into the MCG in adulthood, number of FG-positive neurons in the LS decreased to the male level.2.Serotonergic neurons inhibiting lordosis behavior.Three kinds of HT_<1A> receptor agonist ; +or +-8-OH-DPAT, buspirone, or an antagonist ; WAY-100635 were injected and sexual behavior was observed in estrogen-treated ovariectomized rats. As a result, the agonist suppressed acutely lordosis and the antagonist enhanced lordosis. In addition, other experiment using HPLC showed that contents of serotonin in the forebrain such as the septum, preoptic area and ventromedial hypothalamus of the females were higher than those in male rats.3. Sex difference of maternal-like behavior in juvenile rats.Observation of maternal behavior in juvenile rats showed 20 days-age male rats show retrieving behavior more frequently than females of same age. Incidence of retrieving behavior decreased on 30 days after birth. Injection of estrogen on day 5 after birth in female rats increased incidence of retrieving to the male level on day 20

1.对侧脑室系统的前凸抑制作用将荧光金（Fluoro-Gold，FG）逆行示踪剂注入雄性和雌性大鼠的中脑中央灰质（MCG），免疫组织化学法检测FG、外侧隔（LS）内雌激素α和β受体的表达。结果表明：（1）雌性大鼠LS中FG阳性细胞数多于雄性大鼠;（2）LS中β受体免疫阳性细胞数多于α受体免疫阳性细胞。外源性雌激素不影响这些细胞含雌激素β和α受体的数量。（3）LS内FG阳性神经元中β受体阳性细胞的比例，雌雄大鼠均小于20%。雌性大鼠生后5天注射苯甲酸雌二醇（EB），成年后MCG注射FG，免疫组化显示LS内FG阳性神经元数降至雄性水平。2.抑制脊柱前凸行为的5-羟色胺能神经元<1A>。注射WAY-100635，观察雌激素处理的卵巢切除大鼠的性行为。因此，激动剂抑制急性脊柱前凸和拮抗剂增强脊柱前凸。此外，HPLC实验还显示，雌性大鼠前脑隔区、视前区和下丘脑腹内侧区5-羟色胺含量高于雄性大鼠.幼年大鼠母性行为的性别差异对幼年大鼠母性行为的观察发现，20日龄雄性大鼠比同龄雌性大鼠更易出现母性行为。出生后30天，检索行为发生率下降。雌鼠出生后第5天注射雌激素可增加第20天恢复到雄性水平的发生率

项目成果

Facilitatory effects of QAY-100635,a 5-HT_<1A> receptor antagonist, on lordosis in female rats.

QAY-100635（一种5-HT_1A 受体拮抗剂）对雌性大鼠脊柱前凸的促进作用。

• 发表时间: 2004
• 期刊: Neurosci.Lett. 371(2-3)
• 作者: Kishitake;M.;Ymanaouchi;K.
• 通讯作者: K.

Postnatal apotosis, development, and sex difference in the lateral septum of rats

大鼠出生后外隔膜的凋亡、发育和性别差异

• 发表时间: 2004
• 期刊: J.Comp.Neurol. 475, 2
• 作者: Tsukahara;S.;et al.
• 通讯作者: et al.

歯の人間科学

人类牙齿科学

• 发表时间: 2003
• 作者: Kakeyama;M.;Umino;A.;Nishikawa;T.;Yamanouchi;K.;山内兄人(編著);山内兄人(分担執筆);山内兄人(分担執筆);山内兄人
• 通讯作者: 山内兄人

Facilitatory effects of QAY-100635, a 5-HT1A receptor antagonist, on lordosis in female rats.

QAY-100635（一种 5-HT1A 受体拮抗剂）对雌性大鼠脊柱前凸的促进作用。

• 发表时间: 2004
• 期刊: Neurosci, Lett. 371
• 作者: Kishitake;M.;Yamanouchi;K.
• 通讯作者: K.

Postnatal apoptosis, development, and sex difference in the lateral septum of rats

大鼠侧隔膜出生后细胞凋亡、发育及性别差异

• 发表时间: 2004
• 期刊: J.Comp.Neurol. 475
• 作者: Tsukahara;S.;inami;K.;Maekawa;F.;Kakeyama;M.;Yokoyama;T.;Yuji;M.Kitagawa;H.;Kannan;Y.;Yamanouchi;K.
• 通讯作者: K.