Neural regulatory systems for sexual and maternal behaviors and the sexual differentiatio

1.将逆行示踪剂（Fluoro-Gold， FG）注射到雄性和雌性大鼠的中脑中央灰区（MCG），并对FG、雌激素a和外侧隔区(LS) β受体进行免疫组化分析。结果表明：(1)雌鼠LS中fg阳性细胞数量多于雄性；(2)与a受体免疫阳性细胞相比，雌鼠LS中β受体免疫阳性细胞较多。含有雌激素β和α受体的细胞数量不受外源雌激素的影响。(3)雌雄大鼠LS中FG阳性神经元含β受体细胞的比例均小于20%。雌性大鼠出生后第5天注射雌二醇（estradiol benzoate， EB），成年后在MCG注射FG后进行免疫组化分析，LS中FG阳性神经元数量减少至雄性水平2。5 -羟色胺能神经元抑制前凸行为。三种HT_<1A>受体激动剂；+或+-8-OH-DPAT、丁螺环酮或拮抗剂；注射WAY-100635，观察雌激素处理的去卵巢大鼠的性行为。结果，激动剂急性抑制前凸，而拮抗剂增强前凸。此外，其他HPLC实验显示，雌性大鼠的前脑如中隔、视前区和下丘脑腹内侧的血清素含量高于雄性大鼠。幼年大鼠母性行为的性别差异。对幼年大鼠母性行为的观察发现，20日龄雄性大鼠比同龄雌性大鼠表现出更频繁的寻回行为。在出生后30天，取回行为的发生率下降。雌性大鼠出生后第5天注射雌激素，可使雌性大鼠在第20天恢复到雄性大鼠的水平

1.lordosis-inhibitiory influence in the lateral systemThe retrograde tracer (Fluoro-Gold, FG) was injected into the midbrain central gray(MCG) and immunohistochemical analysis for FG, estrogen a and βreceptors in the lateral septum(LS) was performed in male and female rats. As the results, (1)Number of FG-positive cells in the LS in females was larger than that in males, (2)Numerous βreceptor-immunopositive cells were found in the LS, compared to the a receptor immunopositive cells. Number of these cells containing estrogen β and α receptors was not influenced by exogenous estrogen. (3)Rate of β receptor containing cells in FG positive neurons in the LS was less than 20% in both male and female rats. When estradiol benzoate(EB) was injected to female rats on day 5 after birth and immunohistochemical analysis was performed after injection of FG into the MCG in adulthood, number of FG-positive neurons in the LS decreased to the male level.2.Serotonergic neurons inhibiting lordosis behavior.Three kinds of HT_<1A> receptor agonist ; +or +-8-OH-DPAT, buspirone, or an antagonist ; WAY-100635 were injected and sexual behavior was observed in estrogen-treated ovariectomized rats. As a result, the agonist suppressed acutely lordosis and the antagonist enhanced lordosis. In addition, other experiment using HPLC showed that contents of serotonin in the forebrain such as the septum, preoptic area and ventromedial hypothalamus of the females were higher than those in male rats.3. Sex difference of maternal-like behavior in juvenile rats.Observation of maternal behavior in juvenile rats showed 20 days-age male rats show retrieving behavior more frequently than females of same age. Incidence of retrieving behavior decreased on 30 days after birth. Injection of estrogen on day 5 after birth in female rats increased incidence of retrieving to the male level on day 20