Design of Differential Pressure Detection System for Size Exclusion Chromatography and the Estimation of its Performance

尺寸排阻色谱差压检测系统的设计及其性能评估

• 批准号: 63550565
• 负责人: MORI Sadao
• 金额: $ 1.54万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63550565/
• 关键词: Differential pressure detection system; Determination of molecular weight; Size exclusion chromatography; Polymer analysis; High-performance liquid chromatography; Calillary viscometer; サイズ排除クロマトグラフィー; 高分子物質; 固有粘度

When a polymer solution passes through a capillary tubing, pressure difference between the inlet and the outlet of the capillary tubing is proportional to the viscosity of the polymer solution which changes with molecular weight as well as the concentration. A differential pressure detection system was designed and the system was applied to the polymer solution eluted from size exclusion chromatography (SEC) columns.To calculate molecular weight averages for polymers, a calibration curve of log RpxM vs retention volume was constructed using polystyrene (PS) samples. Here, Rp is the value of the differential pressure divided by the response of a differential refractometer (RI) and M is molecular weight. A correction procedure of the time lag of the response of RI was developed. To apply the calibration curve constructed with PS to polymers other than PS, response correction factors for these polymers were measured and molecular weight averages of these polymers were determined accurately.This system was also applied for the determination of molecular weight distributions and composition distributions for several copolymers.

当聚合物溶液通过毛细管时，毛细管入口和出口之间的压差与聚合物溶液的粘度成正比，聚合物溶液的粘度随分子量以及浓度而变化。设计了一种差压检测系统，并将该系统应用于从尺寸排阻色谱（SEC）柱上洗脱的聚合物溶液中，以聚苯乙烯（PS）样品构建log RpxM与保留体积的校准曲线，计算聚合物的平均分子量。这里，Rp是压差除以差示折射计（RI）的响应的值，M是分子量。开发了RI响应时间滞后的校正程序。为了将用PS构建的校准曲线应用于PS以外的聚合物，测定了这些聚合物的响应校正因子，准确测定了这些聚合物的平均分子量，并将该系统应用于几种共聚物的分子量分布和组成分布的测定。

项目成果

森定雄: "Liquid Adsorption Chromatography and Size Exclusion Chromatography of Styrene-Methyl Methacrylate Copolymers" Journal of Applied Polymer Science;Applied Polymer Symposium. 43. 65-84 (1989)

Sadao Mori：“苯乙烯-甲基丙烯酸甲酯共​​聚物的液体吸附色谱法和尺寸排阻色谱法”《应用聚合物科学杂志》；应用聚合物研讨会 43. 65-84 (1989)。

Sadao Mori: "Determination of Chemical Composition Distribution as a Function of Molecular Size for Poly(styrene-methyl methacrylate) Random Copolymers by Liquid Adsorption Chromatography and Size Exclusion chromatography" Anal. Sci., 4, 365-369, 1988.

Sadao Mori：“通过液体吸附色谱法和尺寸排阻色谱法测定聚（苯乙烯-甲基丙烯酸甲酯）无规共聚物的化学成分分布与分子大小的函数关系”。

森定雄: "Size Exclusion Chromatography of Poly(ethylene・Tere-phtha late)Using Hexafluoro-2-Propanal as the Mobile phase" Analytical Chemistry. 61. 1321-1325 (1989)

Sadao Mori：“使用六氟-2-丙醛作为流动相的聚（乙烯·对苯二甲酸酯）的尺寸排阻色谱法”分析化学 61. 1321-1325 (1989)

Sadao Mori: "Size Exclusion Chromatography of Poly(ethylene terephthalate) Using Hexafluoro-2-propanol as the Mobile Phase" Anal. Chem., 61, 1321-1325, 1989.

Sadao Mori：“使用六氟-2-丙醇作为流动相的聚对苯二甲酸乙二醇酯的尺寸排阻色谱法”分析。

Sadao Mori, Masaaki Mouri: "Separation of Styrene-Acrylate and Styrene-Methacrylate Copolymers According to Composition by Liquid Adsorption Chromatography" Anal. Chem., 61, 2171-2175, 1989.

Sadao Mori、Masaaki Mouri：“通过液体吸附色谱法根据组成分离苯乙烯-丙烯酸酯和苯乙烯-甲基丙烯酸酯共聚物”。