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Development of a gel permeation chromatography method for the determination of molecular weight of bacterial cellulose and cellulose nanocrystals

开发凝胶渗透色谱法测定细菌纤维素和纤维素纳米晶体的分子量

基本信息

批准号：
492836-2015
负责人：
Wan, Wankei
金额：
$ 1.82万
依托单位：
University of Western Ontario
依托单位国家：
加拿大
项目类别：
Engage Grants Program
财政年份：
2016
资助国家：
加拿大
起止时间：
2016-01-01 至 2017-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=602219
关键词：
Development gel permeation chromatography method

项目摘要

Cellulose derived from plant sources is one of the most abundant natural biopolymer and finds applications in many products in our daily life. As a polymer, application is strongly dependent on its molecular weight. Gel permeation chromatography is one of the standard methods for molecular weight determination which requires the dissolution of the polymer to form a solution. However strong hydrogen bonding which hinders cellulose solubility in most solvent systems makes its molecular weight determination difficult. There a few solventsystems that are found to be suitable. Bacterial cellulose, produced by bacteria, is in the form of nanofiber that is substantially pure. Due to its high degree of crystallinity, it is especially challenging to find a suitable solvent system. Similar problem exists for cellulose nanocrystals which are also highly crystalline and difficult to dissolve. As a result, no definitive molecular weight has been determined for bacterial cellulose even though medical devices including replacement dura mater and occlusive wound dressing are already commercialized and many potential biomedical and medical device applications are being pursued. This project will investigate solvent systems for effective bacterial cellulose dissolution and develop a new procedure for molecular weight determination of bacterial cellulose. A range of solvent systems in combination with GPC columns and GPC operating conditions will be investigated. Upon completion of the project, a standard operating procedure using GPC for molecular weight determination of bacterial cellulose and cellulose nanocyrstals will be developed. This procedure can also be applied to conventionally derived plant cellulose.

来源于植物的纤维素是最丰富的天然生物聚合物之一，并在我们日常生活中的许多产品中得到应用。作为一种聚合物，应用强烈依赖于其分子量。凝胶渗透色谱法是分子量测定的标准方法之一，其需要溶解聚合物以形成溶液。然而，强氢键阻碍了纤维素在大多数溶剂体系中的溶解性，使得其分子量的测定变得困难。有几种溶剂体系被认为是合适的.由细菌产生的细菌纤维素是基本上纯的纤维素形式。由于其高结晶度，寻找合适的溶剂系统尤其具有挑战性。对于也是高度结晶且难以溶解的纤维素纳米晶体存在类似的问题。因此，尽管包括替换硬脑膜和闭塞性伤口敷料的医疗器械已经商业化，并且正在追求许多潜在的生物医学和医疗器械应用，但细菌纤维素的分子量尚未确定。本计画将探讨细菌纤维素有效溶解的溶剂系统，并发展一种新的细菌纤维素分子量测定方法。将研究一系列溶剂系统与GPC柱和GPC操作条件的组合。项目完成后，将开发一个使用GPC测定细菌纤维素和纤维素纳米晶体分子量的标准操作程序。该方法也可应用于常规来源的植物纤维素。