Helix Stability and Transition Behavior of Helix Sense of Polypeptide

多肽螺旋链的稳定性和转变行为

• 批准号: 63550663
• 负责人: TSUJITA Yoshiharu
• 金额: $ 1.15万
• 依托单位: Nagoya Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63550663/
• 关键词: Polypeptide; Helix Stability; Helix Sense; alpha Helix; Circular Dichroism; Polyglutamic Acid Ester; Polyaspartic Acid Ester; 円二色正; CD

Polyaspartic acid esters have unstable and reversed helix sense to stable Polyglutamic acid esters because of its competitive hydrogen bond between NH group in backbone and C=O group in side chain. The object of a present study is to clarify helix stability and helix sense of polyaspartic acid esters through the interaction of side chain - backbone and side chain.Various copolyaspartic acid esters were synthesized and its circular dichroism spectra in solution and solid state were examined as a function of composition and temperature. Left handed helix of polyaspartic acid benzyl esters transformed to right handed helix by the replacement of benzyl to alkyl group except for methyl group. Furthermore copolyaspartic acid esters with low alkylation transformed to right handed alpha helix sense with increasing temperature. These behavior is interpreted in terms of the competitive hydrogen bond between backbone and side chain.

聚天冬氨酸酯对稳定的聚谷氨酸酯具有不稳定的反螺旋意义，这是因为其主链上的NH基团与侧链上的C=O基团之间存在竞争性氢键。本研究的目的是通过侧链-主链和侧链的相互作用来阐明聚天冬氨酸酯的螺旋稳定性和螺旋感。合成了多种共聚物天冬氨酸酯，研究了其在溶液和固体状态下的圆二色性光谱与组成和温度的关系。聚天冬氨酸苄基酯的左旋螺旋被除甲基外的烷基取代而转变为右旋螺旋。低烷基化的共聚物天冬氨酸酯随着温度的升高转变为右旋α螺旋形。这些行为可以用主链和侧链之间的竞争性氢键来解释。

项目成果

Y.Tsujita,T.Watanabe,A.Takizawa,T.Kinoshita: "Characterization of Copolyaspartates Containing a Long Alkyl Side Chain" Polymer,in press.

Y.Tsujita、T.Watanabe、A.Takizawa、T.Kinoshita：“含有长烷基侧链的共聚天冬氨酸”聚合物的表征，正在出版。

辻田義治ら: "エッセンシャル高分子科学" 講談社サイエンティフィク, 42 (1988)

Yoshiharu Tsujita 等人：《高分子科学基础》讲谈社科学，42 (1988)

M.Sato,T.Kinoshita,A.Takizawa,Y.Tsujita: "Photoinduced Conformational Transition of Polypeptides Containing Azobenzenesulfonate in the Side Chains" Macromolecules. 21. 1612-1616 (1988)

M.Sato，T.Kinoshita，A.Takizawa，Y.Tsujita：“侧链中含有偶氮苯磺酸盐的多肽的光诱导构象转变”大分子。

辻田義治ら: "アミノ酸ポリマ-ー合成と応用ー" シ-エムシ-, 25 (1988)

Yoshiharu Tsujita 等人：“氨基酸聚合物 - 合成和应用” CMCI，25 (1988)

M. Sato, T. Kinoshita, A. Takizawa, and Y. Tsujita: "Photoinduced Conformational Transition of Polypeptides Containing Azobenzenesulfonate in the Side Chains" Macromolecules, 21(6), 1612-1616, 1988.

M. Sato、T. Kinoshita、A. Takizawa 和 Y. Tsujita：“侧链中含有偶氮苯磺酸盐的多肽的光诱导构象转变”《高分子》，21(6), 1612-1616, 1988。