STUDIES ON GENETIC MANIPULATIONS OF FLOWER COLOR USING PLANT PROTOPLASTS.

利用植物原生质体对花色进行基因调控的研究。

• 批准号: 63560008
• 负责人: ADACHI Taiji
• 金额: $ 1.28万
• 依托单位: MIYAZAKI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63560008/
• 关键词: Protoplast; flower color gene; betalain pigment; Portulaca; チロシン; 花色; 遺伝分析; 遺伝子交換; ポーチュラカ

In order to develop genetic manipulations in crops by means of biotechnology, we have to investigate with a model system. Biogenesis of betalain pigments is available not only for a genetical analysis of flower color but also for a biotechnological approach to secondary metabolites in plants. Portulaca is acceptable as an ideal plant which allows 1) genetical analysis, 2) in vitro culture and 3) interesting secondary metabolites. In this project, protoplast isolation was established with some parts of plant in the genus Portulaca. Micro analytical method was developed for testing betalain biogenesis during tissue culture. The actions of four genes were set up in the biosynthetic process of betalains. These genotypes were compared with protein constitutions during developmental stages. Some target proteins were specified, but further analysis and genetic approach for improvement were still remained

为了利用生物技术对作物进行遗传操作，我们必须用模型系统进行研究。甜菜色素的生物发生不仅可用于花色的遗传分析，而且可用于植物次生代谢产物的生物技术方法。马齿苋是一种理想的植物，可以进行1）遗传分析，2）离体培养和3）感兴趣的次生代谢产物。本研究以马齿苋属植物的部分器官为材料，建立了原生质体分离的方法。建立了甜菜色素组织培养过程中生物合成的微量分析方法。在甜菜色素的生物合成过程中，建立了四个基因的作用机制。这些基因型与蛋白质组成在发育阶段进行了比较。目前已确定了一些靶蛋白，但仍需进一步的分析和遗传改良

项目成果

貴島祐治: "マツバボタンにおけるベタレイン系色素前駆体の解析I異花色同質遺伝子系統の比較" 育種学雑誌. 39(別2). 350-351 (1989)

Yuji Kishima：“马齿苋中甜菜碱色素前体的分析 I。不同花色的同基因系的比较”《育种科学杂志》39（第 2 部分）（1989 年）。

KISHIMA, Y. and T. ADACHI: "Genetic analysis for biogenesis of betalains and its precursor in Portulaca. I the comparison of components in isogenic lines. (in japanese)" Japan. J. Breed. vol 39 (supp.2) 350-351 1989.

KISHIMA, Y. 和 T. ADACHI：“马齿苋中甜菜碱及其前体生物发生的遗传分析。I 同基因系中成分的比较。（日语）”日本。

足立泰二: "ポ-チュラカ属植物の花蕾発育過程におけるベタレイン色素とタンパク質の変異" 遺伝学雑誌. 63. 549 (1988)

Taiji Adachi：“马齿苋属植物花芽发育过程中甜菜碱色素和蛋白质的突变”《遗传学杂志》63. 549 (1988)。

ADACHI, T., K. NAKAMURA and H. YOSHIDOME: "Biogenesis of Betalain pigments and Gene Expression during development of flower bud in Portulaca. (in japanese)" Japan. J. Breed.vol 38 (suppl.1). 418-419 (1988)

ADACHI, T.、K. NAKAMURA 和 H. YOSHIDOME：“马齿苋花芽发育过程中甜菜红素色素的生物发生和基因表达。（日语）”日本。

T.Adachi.;Kishima.Y.;Suilo.M.;Genetic control of Betalain pigment during development of flower buds in Portulaca: 30(Suppl). 1988. (456)

T.Adachi.；Kishima.Y.；Suilo.M.；马齿苋花芽发育过程中甜菜红素色素的遗传控制：30（增刊）。