权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Immunohistological study of the developing peripheral of chicken with use of a whole-mount staining method

使用整体染色法对鸡外周发育的免疫组织学研究

基本信息

批准号：
63570028
负责人：
TANAKE Shigenori
金额：
$ 1.6万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570028/
关键词：
peripheral nerve development immunohistochemistry 末梢神経ニワトリ免疫染色

项目摘要

Chick embryos at stage 13 to 35 were immunohistochemically stained in whole-mount using anti-neurofilament antibody, and the peripheral nerves and their branches, even of finest calibre were traced, and their three-dimentional relationships with the developing aortic arches, the heart and lungs were elucidated.In chick embryos at early stage, e. g. 18 the opthalmic nerve revealed more more remarkable configuration than other two --- i. e., the maxillary and mandibular nerves do. The trigeminal ganglion was immunohistochemically stained at the base of the "tri-furcation" of this nerve, in a reticular network of the neurite. An ectopic trigeminal ganglion was also found not far form this ordinary ganglion, but in the epidermous layer and not under the epidermis (see Anat and Embryol). Branches of the developing vagal nerve and their three-dimentional relationships to the developing lungs, the aortic arch and especially, to the developing heart were thoroughly observed. We have demonstrated clearly that the vagal cardiac branches could be classified into a porta-arteriarum-cardiac branches and a porta-venarum-cardiac branches. The former branches revealed their asymmetric dis-position in accompany with the asymmetric disposition of the aortic arches : At stage 23-25, the 4th and 6th arches rotate in a counter-clock-wise direction, rendering the 4th aortic arch is located ventrally, while the 6th aortic arch located dorsally. Concomitantly with this rotation of the aortic arches, the left porta-arteriarum-cardiac branch become located ventrally, while the right porta-arteriarum-cardiac branch located dorsally. (see Anat and Embryol). The latter cardiac branches found to enter the heart in accompany with the pulmonary veins--via porta venarum.

用抗神经丝抗体对13 ~ 35期鸡胚进行整体包埋化学染色，追踪周围神经及其分支，并阐明其与发育中的主动脉弓、心脏和肺的三维关系。G. 18.视神经的形态较其他两种更为显著。例如，上颌骨和下颌骨的神经有三叉神经节在三叉神经根分叉的基部，在神经突的网状网络中被化学染色。异位三叉神经节也被发现离这个普通的神经节不远，但在表皮层，而不是在表皮下（见Anat和Embryol）。详细观察了迷走神经的分支及其与肺、主动脉弓、特别是心脏的三维关系。我们已经清楚地证明，迷走神经心脏支可分为动脉门心脏支和静脉门心脏支。在23-25期，第4、6主动脉弓呈逆时针方向旋转，第4主动脉弓位于腹侧，第6主动脉弓位于背侧。伴随着主动脉弓的这种旋转，左门-动脉-心脏分支变得位于腹侧，而右门-动脉-心脏分支位于背侧。(see Anat和Embryol）。发现后一种心支与肺静脉一起通过静脉门进入心脏。