Studies of Neuronal Properties in the Reaggregate Cultures of Rat Retinane

视网膜复合培养取新生大鼠建立。所有的实验都是在压盘后10-15天进行的。组织学研究表明，选择性层的形成在堆积物中重新形成。由于这些组织学特性与体内相似，因此进行了电生理实验，以研究体外可能存在的神经网络，这可能是视网膜功能的基础。可以在最外层的单元上建立单元附着配置。在黑暗条件下，最外层约五分之一的细胞显示自发放电。有趣的是，当光刺激整个神经元时，少数神经元增加了放电频率。具有这种光反应样活性的细胞比例为1.6%。由于免疫组织学研究已经报道，最外层的细胞大部分是无长突细胞，光感受器仅定位于中央区域，这些结果表明重组培养中的光感受器和无长突细胞之间的功能联系重新构建。这些结果还表明，重组培养可以为研究神经网络的体外发育提供有趣的模型。

Retinal reaggregate cultures were established from new born rats. All the experiments were carried out 10-15 days after plating.Histological studies revealed that selective layer formation were reconstituted in the reaggegates. Since these histological properties resembled those in vivo, electro-physiological experiments were carried out in order to study the possible presence of neural net works in vitro, which might be rudiMentary for retinal functions. It was possible to establish cell attach configuration on the cells in the outermost layer. Under a dark condition, about one fifth of the cells in the outermost layer showed spontaneous discharges. Interestingly, a few neurons increased the frequency of discharges when light stimuli was given to the whole reaggregate. The proportion of the cells with such light-response like activity was 1.6%. Since it was already reported by an immunohistological study that most cells in the outermost layer were amacrine cells and the photoreceptors were exclusively localized in the central region, these results indicated that functional connections were reconstituted between the photoreceptors and the amacrine cells in the reaggregate cultures. These results also demonstrated that the reaggregate cultures could provide interesting models to study the development of neural networks in vitro.