STUDY OF A NEURON-SPECIFIC PROTEIN,HPC-1, WHICH REGULATES NEURONAL SPROUTING.

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This research project was carried out in order to study the physiological role of HPC-1/Syntaxin 1A.Administration of the antibody against HPC-1 resulted in increase of catechoilamine release from PC12 cells and transmitter release from autosynapse of cultured hippocampal neurons. Overexpression of HPC-1 in beta TC cell line caused decrease of insulin secretion. These results suggested that HPC-1 playd important role in regulated secratory process as a negative regulator. In dorsal root ganglion cells and cerebellar granular cells in vitro, administration of the antisense oligonucleotide against HPC-1 mRNA enhanced neurite sprouting within 12 hours. Transfection experiments with a vector carrying the antisense RNA to PC12 cells showed that suppression of HPC-1 expression caused increase of catecholamin release and enhancement of small process formation, clearly indicating the multifunctional role of HPC-1. Expression of HPC-1 gene in hippocampal neurons was suppressed by kainic acid.