Effects of Protease from Plerocercoid of Spirometra erinacei on Host Structural Protein.

猴头螺旋体蛋白酶对宿主结构蛋白的影响。

• 批准号: 63570182
• 负责人: YANAGISAWA Toshio
• 金额: $ 1.34万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570182/
• 关键词: Spirometra erinacei; Plerocercoid; Cysteine protease; マンソン裂頭条虫; プレロセルコイド; 孤虫症; チオールプロテアーゼ

Thiol protease was isolated from plerocercoid of Spirometra erinacei (plerocercoid protease, pl-p). Splitting activities of the protease against structural and contractile proteins from host vertebrate skeletal muscle were investigated. the results obtained are as follows.1) Pl-P may cleave native actin and myosin peptides. G-actin is digested by pl-p more quickly than F-actin. ATPase activity remained intact on the residues produced by myosin digestion.2) A myofibril prepared from host skeletal muscle, having macromolecular structure may also digested by pl-p in vitro. myosin, a constituent of the thick-filament on, myofibril, is splitted and dissolved without a loss of its ATPase activity.3) Effects of pl-p on the host actomyosin containing a regulatory system (myosin-B) were examined by changes in superprecipitation as an in vitro model of muscular contraction. Ca^<2+>-sensitivity in the actomyosin system is lost by the addition of pl-p.4) The effect of pl-p on regulatory proteins, tropomyosin and troponin was examined. Substrates used are cleaved more preferably cleaved by pl-p in the following order: troponin T-subunit<greater than or equal> troponin I-subunit > tropomyosin > troponin CResults obtained from the present study suggest that pl-p from S. erinacei plerocercoids may be able to degradate host muscular structure.

从埃里温迭宫绦虫（Spirometra erinacei）的裂头蚴中分离到巯基蛋白酶（plerocercoid protease，pl-p）。研究了该蛋白酶对宿主脊椎动物骨骼肌结构蛋白和收缩蛋白的裂解活性。结果表明：1）P1-P能切割天然肌动蛋白和肌球蛋白肽。G-肌动蛋白比F-肌动蛋白更快地被pl-p消化。2）从宿主骨骼肌中提取的具有大分子结构的肌原纤维在体外也能被pl-p消化。肌球蛋白是肌原纤维上的粗丝的一种成分，它被分裂和溶解而不丧失其ATP酶活性。3）通过超沉淀的变化作为肌肉收缩的体外模型来检测pl-p对含有调节系统（肌球蛋白-B）的宿主肌动球蛋白的影响。加入pl-p可使肌动球蛋白系统的Ca^<2+>敏感性丧失。4）检测pl-p对调节蛋白原肌球蛋白和肌钙蛋白的影响。所用底物更优选被pl-p以下列顺序切割：肌钙蛋白T-亚基<greater than or equal>肌钙蛋白I-亚基&gt;原肌球蛋白&gt;肌钙蛋白C。erinacei plerocercoids可能能够降解宿主的肌肉结构。