Studies on the growth hormone-like substance produced by plerocercoid of Spirometra erinaceieuropaei.

猴头螺旋体产生的类生长激素物质的研究。

• 批准号: 06454199
• 负责人: HIRAI Kazumitsu
• 金额: $ 2.56万
• 依托单位: TOTTORI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454199/
• 关键词: Spirometra erinaceieuropaei; Plerocercoid; Growth factor; Cathepsin-L like substance; Growth hormone; Host-parasite relationship; 擬充尾虫; 宿主-寄生体関係; システインプロテイナーゼ

We conducted various experiments to clarify properties of the growth hormone (GH)-like substance produced by plerocercoids of Spirometra erinaceieuropaei, and got results as follows :1. The worm extract creavaged the anti-27kDa protein antibody as antibody against GH-like substance, when the worm extract was incubated with the biotinylated anti-27kDa protein antibody.2. This ability of the extract was inhibited by addition of cystein proteinase inhibitors such as E64 and leupeptin.3. The ^<125>I-anti-27kDa proten antibody was incorporated into plerocercoid bodidy when plerocercoids were cultured with ^<125>I-anti-27kDa protein antibody and incorporation of antibody was enhanced with increase in antibody added and duration of culture.4. Murine macrophages activated by the excretory/secretory (ES) products of plerocercoids expressed the mRNAs of TNF-alpha and IL-65. Macrophages cultured with ES products were suppressed expression of nitric oxide synthase, TNF-alpha and IL-6 mRNA in LPS-activated macrophages but enhanced expression of IL-6 mRNA in IFN-gamma activated macrophages.These results suggest that 27 kDa protein and other products produced by plerocercoids inhibit humoral and cellural immunity of the host.

本研究通过多项实验对刺猬迭宫绦虫多尾蚴产生的生长激素样物质的性质进行了研究，主要结果如下：1。当蠕虫提取物与生物素化的抗27 kDa蛋白抗体孵育时，蠕虫提取物产生抗27 kDa蛋白抗体作为抗GH样物质的抗体.半胱氨酸蛋白酶抑制剂如E64和亮抑酶素的加入抑制了提取物的这种能力.用<125>抗-27 <125>kDa蛋白抗体培养类尾蚴，可使抗-27 kDa蛋白抗体掺入类尾蚴体内，抗体掺入量随抗体加入量的增加和培养时间的延长而增加.由类尾蚴排泄/分泌（ES）产物激活的小鼠巨噬细胞表达TNF-α和IL-65的mRNA。ES产物抑制LPS激活的巨噬细胞中一氧化氮合酶、TNF-α和IL-6 mRNA的表达，而增强IFN-γ激活的巨噬细胞中IL-6 mRNA的表达，提示27 kDa蛋白和ES产物抑制宿主的体液免疫和细胞免疫。

项目成果

Hao-Ran, WANG: "Immunohistochemical localization of 27kDa protein in the plerocercoids of Spirometra erinacei" Japanese Journal of Parasitology. 44. 6-11 (1995)

王浩然：“猴头螺旋体中 27kDa 蛋白的免疫组织化学定位”，《日本寄生虫学杂志》。

T.TUBOI et al.: "Hepatotrophic activity in mouse serum infected with plerosercoids of Spirometra erinacei." Parasitol.Res.80. 629-633 (1994)

T.TUBOI 等人：“感染了猴头螺旋体的 plerosercoids 的小鼠血清中的保肝活性。”

TaKaFuMI, TSUBOI: "Hepatotrophic activity in mouse serum infected with plerocercaids of Spirometra erinacei." Parasitology Research. 80. 629-633 (1994)

TaKaFuMI、TSUBOI：“感染了猴头螺旋体的小鼠血清中的保肝活性。”

Takafumi TSUBOI: "Hepatotrophic activity in mouse serum infected with plerocercoids of Spirometra erinacei." Parasitology Research. 80. 629-633 (1994)

Takafumi TSUBOI：“感染了猴头螺旋体的小鼠血清中的保肝活性。”

Soji, FUKUMOTO: "Killing of newborn Trichinella spiralis larvae by macrophage Cell line." Yonago Acta Medica. 37. 219-225 (1994)

Soji, FUKUMOTO：“巨噬细胞系杀死新生旋毛虫幼虫。”