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Studies on the growth hormone-like substance produced by plerocercoid of Spirometra erinacei.

猴头螺旋体产生类生长激素物质的研究。

基本信息

批准号：
03670193
负责人：
HIRAI Kazumitsu
金额：
$ 1.34万
依托单位：
Tottori University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

项目摘要

To investigate propaties of the grwth hrmne(GH)-like substnce produced by plerocercoids of Spirometra erinacei, we conducted various experiments and these results were as follows :1. The GH-like substance bound the GH binding protein purified from the rabbit serum, but did not bind it from human serum when the binding ability was observed using radioreceptor assay for GH.2. The plerocercoid extract and 27kd protein, which was purified from plerocercoid extract as GH-like factor and was cystein proteinase-like factor, exhibited the cleaving ability of the biotinylated human IgG antibody and this ability was inhibited by cystein proteinase inhibitors such as E64 and leupetine, but not pepstatine.3. The incubation medium of plerocercoids suppressed the antibody-dependent phagocytosis of the established macrophage, A 640-BB2 strain.4. The incubation medium of plerocercoid stimulated NO_2^- synthesis in the interferon^- gamma priming macrophage but markedly suppressed it in the lipoplysaccharide-stimulting macrophages.5.27kD protein existed in the subtegumental cell layr and on the surface of the tegument and then in the shedding part of scolex when existence of 27kD protein was observed by the immunohistchemical method using the monoclonal antibody agaist 27.5kD protein of S.manso noides plerocercoid.In the present study, these results suggest that plerocercoids secrete 27kD protein to escape from the antibody produced by the hst and a substance to supress the NO_2^-synthe sis of macrophages is present in the incubation medium of perocercoids.

为了研究刺猬迭宫绦虫裂头蚴产生的生长激素样物质的性质，我们进行了多项实验，结果如下：1。GH样物质结合从兔血清中纯化的GH结合蛋白，但不结合它从人血清中的结合能力时，观察使用放射受体测定GH。裂头蚴提取物和从裂头蚴提取物中纯化的27 kd蛋白质（GH样因子，半胱氨酸蛋白酶样因子）具有生物素化人IgG抗体的切割能力，这种能力可被半胱氨酸蛋白酶抑制剂如E64和leupetine抑制，但不被胃蛋白酶抑制剂抑制.拟尾蚴孵育液抑制了已建立的巨噬细胞A640-BB 2株的抗体依赖性吞噬作用.在γ-干扰素诱导的巨噬细胞中，类尾蚴的孵育液刺激NO_2 ~-的合成，但在脂多糖诱导的巨噬细胞中明显抑制NO_2 ~-的合成。免疫组化法观察到27 kD蛋白存在时，5.27kD蛋白首先存在于皮层下细胞层，然后出现在皮层表面，最后出现在头节脱落部分本研究结果提示，曼氏血吸虫类裂头蚴分泌27 kD蛋白逃避hst产生的抗体，并提示在类裂头蚴培养液中存在一种抑制巨噬细胞NO_2 ~-合成的物质。