权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Allergological studies on bronchial asthma due to insects - crossreactivity and unique antigenic fraction of insects

昆虫引起的支气管哮喘的变态反应学研究——交叉反应性和昆虫的独特抗原部分

基本信息

批准号：
63570349
负责人：
KINO Toshiya
金额：
$ 1.28万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

1. Studies on antigenic cross-reactivity between extracts of silkworm wing, caddis fly wing, chironomid whole body, and mite, by use of RAST inhibition tests and seasonal fluctuation patterns of IgE antibodies against the above-mentioned antigens. 1) Mutual RAST inhibition tests showed that at high antigenic concentrations, there were almost complete cross-reactibities among silkworm wing, caddis fly wing, and chironomid whole body extracts. But, there was no cross-reactivity between these insects and mite, showing that the allergies due to the insects were independent from mite allergy. 2) Seasonal fluctuation patterns of the IgE antibodies against these insects did not run parallel with each other. This might mean the less cross-reactivity among these insects at clinical levels. These conflicting results promote us to study the antigenic fractions at the molecular basis.2. SDS-PAGE fractionations of the extracts and immunostaining analyses of the unique antigenic fractions. When the insect extracts were reduced by 2-mercaptoethanol and fractionated by SDS-PAGE, at least 16 different bands for silkworm wing, 7 bands for both caddis fly wing and 7 bands for chironomid whole body were detected, of which 75-79K, 67-68K, 63-64K, 40-42K, 32-33K, and 28-30K daltons were reacted to silkworm wing-specific IgE antibodies, 67-68K, 49-50K, and 30-31K daltons were reacted caddis fly-specific IgE antibodies, and 40-42K and 25-27K daltons were reacted to chironomid whole body-specific IgE antibodies, in more than half of our asthmatic patients. These results showed that it was necessary, further, to study the cross-reactivities between these immune-reactive bands of the insects at molecular basis.

1.本文用RAST抑制试验和IgE抗体季节性变化规律，研究了蚕蛹、蚕蛾翅、摇蚊全身和螨类提取物的抗原交叉反应性。1)RAST相互抑制试验表明，在高抗原浓度下，蚕蛹、石蚕蛾翅和摇蚊全身提取物几乎完全交叉反应。但是，这些昆虫与螨之间没有交叉反应，表明昆虫引起的过敏与螨过敏无关。2)季节性波动模式的IgE抗体对这些昆虫没有运行彼此平行。这可能意味着这些昆虫在临床水平上的交叉反应性较低。这些相互矛盾的结果促使我们从分子水平上研究抗原组分.提取物的SDS-PAGE分级和独特抗原级分的免疫染色分析。用2-巯基乙醇还原昆虫提取物，经SDS-PAGE分离，至少检测到16条不同的家蚕翅带、7条不同的鸡翅带和7条不同的摇蚊全身带，其中75- 79 K、67- 68 K、63- 64 K、40- 42 K、32- 33 K和28- 30 K的分子量与家蚕翅特异性IgE抗体反应，在半数以上的哮喘患者中，与石蛾特异性IgE抗体反应的分子量为67- 68 K、49- 50 K和30- 31 K，与摇蚊全身特异性IgE抗体反应的分子量为40- 42 K和25- 27 K。这些结果表明，有必要进一步从分子水平上研究这些免疫反应带之间的交叉反应性。