Allergological studies on bronchial asthma due to insects - crossreactivity and unique antigenic fraction of insects

1.用Rast抑制试验和抗上述抗原的IgE抗体的季节变化规律，研究了蚕翅、蝉蝇翅、摇蚊全身提取物和尘螨提取物之间的抗原交叉反应。1)相互RAST抑制试验表明，在较高的抗原浓度下，蚕翅、蝉蝇翅和摇蚊全身提取物之间几乎完全发生交叉反应。但是，这些昆虫和螨类之间没有交叉反应，这表明由于昆虫引起的过敏是独立于螨类过敏的。2)抗虫IgE抗体的季节消长规律不一致。这可能意味着在临床水平上这些昆虫之间的交叉反应较少。这些相互矛盾的结果促使我们在分子基础上研究抗原组分。提取物的十二烷基硫酸钠-PAGE分级和独特的抗原组分的免疫染色分析。经2-巯基乙醇还原和SDS-PAGE分离后，检测到至少16条不同的蚕翼条带、7条摇蚊全身条带和7条摇蚊全身条带，其中75-79K、67-68K、63-64K、40-42K、32-33K和28-30K的条带与家蚕翅特异性的IgE抗体反应，67-68K、49-50K和30-31K的条带反应，40-42K和25-27K的条带与摇蚊全身特异性的IgE抗体反应。这些结果表明，有必要进一步从分子基础上研究昆虫免疫反应带之间的交叉反应。

1. Studies on antigenic cross-reactivity between extracts of silkworm wing, caddis fly wing, chironomid whole body, and mite, by use of RAST inhibition tests and seasonal fluctuation patterns of IgE antibodies against the above-mentioned antigens. 1) Mutual RAST inhibition tests showed that at high antigenic concentrations, there were almost complete cross-reactibities among silkworm wing, caddis fly wing, and chironomid whole body extracts. But, there was no cross-reactivity between these insects and mite, showing that the allergies due to the insects were independent from mite allergy. 2) Seasonal fluctuation patterns of the IgE antibodies against these insects did not run parallel with each other. This might mean the less cross-reactivity among these insects at clinical levels. These conflicting results promote us to study the antigenic fractions at the molecular basis.2. SDS-PAGE fractionations of the extracts and immunostaining analyses of the unique antigenic fractions. When the insect extracts were reduced by 2-mercaptoethanol and fractionated by SDS-PAGE, at least 16 different bands for silkworm wing, 7 bands for both caddis fly wing and 7 bands for chironomid whole body were detected, of which 75-79K, 67-68K, 63-64K, 40-42K, 32-33K, and 28-30K daltons were reacted to silkworm wing-specific IgE antibodies, 67-68K, 49-50K, and 30-31K daltons were reacted caddis fly-specific IgE antibodies, and 40-42K and 25-27K daltons were reacted to chironomid whole body-specific IgE antibodies, in more than half of our asthmatic patients. These results showed that it was necessary, further, to study the cross-reactivities between these immune-reactive bands of the insects at molecular basis.