Allergological study on insect inhalant allergen-induced bronchial asthma.

昆虫变应原粗提物由多种不同相对分子质量的组分组成，为研究致敏哮喘患者的抗原组分，采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法，将家蚕翅(具有代表性的蛾、蝶变应原)、丝兰蝇翅、摇蚊全身变应原的提取物进行分离，并与分离的昆虫变应原在硝酸纤维素膜上孵育，检测其IgE抗体结合组分。用11例患者血清与等量VILIME混合后的研究结果表明，家蚕翅抗原组分的分子质量分别为68Kd、64Kd、33Kd和2Ikd，叶蜂抗原组分的分子质量分别为68Kd、48Kd、30Kd和14.4Kd，摇蚊全身抗原组分的分子质量分别为64Kd、26Kd和24Kd。

Crude extracts of insect allergens are composed of many components with different molecular weight.In order to study the antigenic fractions that sensitize the patients with bronchial asthma, we fractionated, by use of SDS-PAGE, the extracts of silkworm wing(the representative allergens of moth and butterfly), caddis fly wing, and chironomid whole body, and then examined the IgE antibodybinding fractions after incubating the sera from II patients with bronchial asthma, with the fractionated insect allergens on nitrocellulose membrane.Different antigenic fractions, namely heterogeneous sensitization to each insect, were found among tlie II patients. The results of the study using the serum, mixed with equivalent voILime from tlie 11 patients, showed that the molecular weiglit of silkworm wing -antigenic fractions was 68Kd, 64Kd, 33Kd and 2IKd, that of caddis fly whig -antigenic fractions was 68Kd, 48Kd, 3OKd and 14.4Kd and that of chironomid whole body - antigenic fractions was 64Kd, 26Kd and 24Kd.Almost all of these fractions were absorbed by each insect, but not by mite and the same was observed vice versa.