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Allergological study on insect inhalant allergen-induced bronchial asthma.

昆虫吸入性过敏原诱发支气管哮喘的变态反应学研究。

基本信息

批准号：
02807080
负责人：
KINO Toshiya
金额：
$ 1.02万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

Crude extracts of insect allergens are composed of many components with different molecular weight.In order to study the antigenic fractions that sensitize the patients with bronchial asthma, we fractionated, by use of SDS-PAGE, the extracts of silkworm wing(the representative allergens of moth and butterfly), caddis fly wing, and chironomid whole body, and then examined the IgE antibodybinding fractions after incubating the sera from II patients with bronchial asthma, with the fractionated insect allergens on nitrocellulose membrane.Different antigenic fractions, namely heterogeneous sensitization to each insect, were found among tlie II patients. The results of the study using the serum, mixed with equivalent voILime from tlie 11 patients, showed that the molecular weiglit of silkworm wing -antigenic fractions was 68Kd, 64Kd, 33Kd and 2IKd, that of caddis fly whig -antigenic fractions was 68Kd, 48Kd, 3OKd and 14.4Kd and that of chironomid whole body - antigenic fractions was 64Kd, 26Kd and 24Kd.Almost all of these fractions were absorbed by each insect, but not by mite and the same was observed vice versa.

昆虫变应原粗提物由多种分子量不同的组分组成，为了研究致敏性抗原组分，我们采用SDS-PAGE对蚕蛹提取物进行了分离（蛾类和蝴蝶的代表性过敏原）、石蛾翅、摇蚊全身，然后用硝酸纤维素膜上分离的昆虫变应原与II型支气管哮喘患者血清孵育后，检测IgE抗体结合组分。不同的抗原组分，即对每种昆虫的异质致敏性，在II型患者中发现。用11名患者的血清与等量血清混合进行研究，结果表明，家蚕丝翅抗原组分的分子量为68 Kd、64 Kd、33 Kd和21 Kd，石蛾抗原组分的分子量为68 Kd、48 Kd、30 Kd和14.4 Kd，摇蚊全身抗原组分的分子量为64 Kd，26 Kd和24 Kd，这些组分几乎都被每种昆虫吸收，但不被螨吸收，反之亦然。