Inhibitory Action of Polyamines on Gastrointestinal Motor Function and its Relation to Emesis

多胺对胃肠运动功能的抑制作用及其与呕吐的关系

• 批准号: 63570617
• 负责人: HADA Ryukichi
• 金额: $ 0.83万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570617/
• 关键词: Polyamine; Spermine; Emesis; Vomiting; Calcium antagonism; polyamine; spermine; calcium blocker; calcium entry; rat small intestine; vomiting; migrating motor complex; MMC

Although polyamines are organic cations ubiquitously occurring in living tissues including gastrointestinal (GI) smooth muscle, any possible physiological or pharmalogical role of these amines on GI motor function has scarcely been investigated. We conducted preliminary in-vitro and in-vivo experiments to examine the effects spermine, one of the polyamines, on GI smooth muscle contractions.All in-vitro experiments were performed on longitudinal ileal strips from male Sprague-Dawley rats, which were suspended in a tissue-bath containing a physiological salt solution with 1.25 mM Ca^<2+>. Spontaneous phasic contractions measured isometrically were markedly inhibited by 3_x10^<-4> M spermine. The presence of 6_x10^<-6> M tetrodotoxine or 1_x10^<-5> M propranorol exhibited no effect on the inhibitory effect of spermine. The inhibitory effect, however, was counteracted by raising the calcium concentration from 1.25 to 3.5 mM.In conscious dogs, contractile activity was recorded from strain g … More auge force transducers and the effects of spermine on GI motility was tested at a intravenous dosage of 30-60 umo1/kg/hr. During the digestive period, a dose of 30 umo1/kg/hr exerted a minimal inhibitory effect on the contractility of the stomach and duodenum, while a strong contraction that mimicked the interdigestive phase III activity occurred in the upper jejunum, which propagated aborally. Spermine 60 umo1/kg/hr produced a marked suppression of the gastric and duodenal motor activity followed by repeated vomiting. Before the emesis, a phase III-like contraction had already appeared at the jejunum, propagating to the ileum. The vomiting ceased with suspension of the spermine infusion, when the entire GI tract took a transient but definite interdigestive pattern. When spermine was administered during the interdigestive period, it evoked repeated emesis but the recycling pattern of migrating motor complex was not essentially disturbed.Spermine might be involved in the controlling mechanism of the GI motor function and in the mechanism of emesis. Less

虽然多胺是有机阳离子普遍存在于活组织，包括胃肠（GI）平滑肌，任何可能的生理或药理作用，这些胺对胃肠运动功能几乎没有调查。我们进行了初步的体外和体内实验来检测精胺（多胺之一）对胃肠道平滑肌收缩的影响，所有体外实验都是在雄性Sprague-Dawley大鼠的纵向回肠条上进行的，这些回肠条悬浮在含有1.25 mM Ca^2+的生理盐溶液的组织浴中。3 × 10 ^ M精胺可显著抑制等距测量的自发性阶段性收缩<-4>。6_x10^<-6>M河豚毒素或1_x10^<-5>M普萘洛尔的存在对精胺的抑制作用没有影响。然而，将钙浓度从1.25 mM提高到3.5 mM时，抑制作用被抵消。 ...更多信息 在消化期间，30 μ mol/kg/hr的剂量对胃和十二指肠的收缩性产生最小的抑制作用，而在上部空肠中发生模仿消化间期III期活动的强烈收缩，其在流产中传播。精胺60 umo 1/kg/hr对胃和十二指肠运动活动产生明显抑制，随后出现反复呕吐。在呕吐之前，空肠已经出现了III期样收缩，并传播到回肠。当整个胃肠道出现短暂但明确的消化间期模式时，暂停精胺输注后呕吐停止。精胺在消化间期给药引起反复呕吐，但对移行性复合运动的再循环模式无实质性影响，精胺可能参与胃肠道运动功能的控制机制和呕吐的发生机制。少

项目成果

Ryukichi,Hada: The Hirosaki Medical Journal. 40. 590-594 (1988)

龙吉，哈达：弘前医学杂志。

Ryukichi Hada: "Inhibitory effect of spermine on the contraction of rat small intestine" The Hirosaki Medical Journal.

Ryukichi Hada：“精胺对大鼠小肠收缩的抑制作用”弘前医学杂志。

Ryukichi Hada: "Spermine fails to inhibit gastric acid secretion in histamine-stimulated pylorus-ligated rats" The Hirosaki Medical Journal. 40. 590-594 (1988)

Ryukichi Hada：“精胺无法抑制组胺刺激的幽门结扎大鼠的胃酸分泌”《弘前医学杂志》。

Ryukichi Hada: "Spermine fails to inhibit gastric secretion in histamineーstimulated pylorusーligated rats." The Hirosaki Medical Journal. 40. 590-594 (1988)

Ryukichi Hada：“精胺无法抑制组胺刺激的幽门结扎大鼠的胃分泌。”《弘前医学杂志》40. 590-594 (1988)。

Ryukichi Hada: "Spermine induces vomiting in the dog" Digestive Disease and Science.

哈达龙吉：“精胺会导致狗呕吐”《消化系统疾病与科学》。