Experimental study on intraoperative diagnosis of metastatic region

术中诊断转移灶的实验研究

• 批准号: 63570649
• 负责人: TAKAMI Hiroshi
• 金额: $ 1.41万
• 依托单位: Teikyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570649/
• 关键词: Monoclonal antibody; Immunoscintigram; 免疫シンチグラフィ-; 免疫シンチグラフィ; モノクローナル抗体; 免疫シンチグラフィー

The localization of 82k/6.3 and 61k/7.5 was investigated by subcellular fractionation. They were rich in Microsomal fraction, but not found in both nuclear and mitochondrial fractions.In binding reaction with seven kinds of lectins, 82k/6.3 reacted with RCA I, DBA and WGA, where 61k/7.5 reacted with RCA I, DBA,WGA,UEA I and SBA. Transferrin:, reacted with only RCA I.Each hybrid producing monoclonal antibody against 82k/6.3 or 61k/7.5 was generated by fusing spleen cell of BALB/C mice immunized by the two proteins and mouse myeloma cells. Each monoclonal antibody was specified in enzyme-linked immunoassay.In indirect immune-fluorescent studies, monoclonal antibodies against 82k/6.3 and 61k/7.5 reacted with cytoplasma and membrane of human cancer cell. This result strongly suggests the localization of the two proteins demonstrated by subcellular fractionation.After iv administration of isotope-labelled monoclonal antibody to tumor- bearing nude mouse, immunoscintigram was taken on i, 3,5 day. Because of no block by iodine, thyroid was accumulated by large amount of iodine. However, implanted tumor at right thigh showed also large amount of accumulation of iodine. Ratio of Tumor/heart blood was 1.46 in 1st day, 1.88<plus-minus>0.21 on 3rd day and 2.36<plus-minus>0.89 on 5th day. This result was promising to apply to clinical investigat

用亚细胞分离法研究了82 k/6.3和61 k/7.5的定位。在与7种凝集素的结合反应中，82 k/6.3与RCA I、DBA和WGA反应，61 k/7.5与RCA I、DBA、WGA、UEA I和SBA反应。转铁蛋白（Transferrin）：仅与RCA Ⅰ反应。将这两种蛋白免疫BALB/C小鼠，取脾细胞与小鼠骨髓瘤细胞融合，分别产生抗82 k/6.3和61 k/7.5的单克隆抗体。在间接免疫荧光研究中，抗82 k/6.3和61 k/7.5单克隆抗体分别与人癌细胞胞浆和胞膜发生反应。将同位素标记的单克隆抗体静脉注射给荷瘤裸鼠，于第1、3、5天取免疫荧光图。甲状腺由于不受碘的阻断，大量碘蓄积。然而，右大腿的植入肿瘤也显示大量碘积聚。第1天瘤心比为1.46，第3天为1.88 <plus-minus>± 0.21，第5天为2.36 <plus-minus>± 0.89。这一结果有望应用于临床

项目成果

Okuda, K: "Biological and immunological studies on human tumor-associated cellular proteins detected by two-dimensional gel electrophoresis" Nippon Geka Gakaishi, 90-10 1697-1705, 1989.

Okuda, K：“通过二维凝胶电泳检测人类肿瘤相关细胞蛋白的生物学和免疫学研究”Nippon Geka Gakaishi，90-10 1697-1705，1989。

奥田康一: "2次元電気泳動により検出されたヒト腫瘍関連細胞蛋白質に関する生化学的ならびに免疫学的研究" 日本外科学会雑誌. 90. 1697-1705 (1989)

Koichi Okuda：“通过二维电泳检测人类肿瘤相关细胞蛋白的生化和免疫学研究”日本外科学会杂志 90. 1697-1705 (1989)。

奥田康一: 日本外科学会雑誌. 90. (1989)

奥田浩一：日本外科学会杂志 90。（1989）

Makamura,K: "Radioimmunodetection of human pancreatic tumor xenografts using DV-PAN II monocloral antibody" Annals of Nuclear Medicine. 2. 1-6 (1988)

Makamura,K：“使用 DV-PAN II 单克隆抗体对人胰腺肿瘤异种移植物进行放射免疫检测”核医学年鉴。

Nakamura, K: "Raioimmunodetection of human pancreatic tumor xenografts using DU-PAN 2 monoclonal antibody" Annals of Nuclear Medicine 2-1, 1-6, 1988.

Nakamura, K：“使用 DU-PAN 2 单克隆抗体对人胰腺肿瘤异种移植物进行放射免疫检测”《核医学年鉴》2-1、1-6，1988 年。