Single high-dose radiotherapy for experimental rat prolactinoma

单次大剂量放疗治疗实验性大鼠泌乳素瘤

• 批准号: 11671353
• 负责人: TAKAHASHI Akio
• 金额: $ 2.24万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671353/
• 关键词: experimental rat pituitary adenoma; single high-dose radiotherapy; prolactinoma; 1回大線量照射; prolactinoma; radiosurgery; diethylstilbestrol; rat

Background : Single high-dose radiotherapy, i.e. gamma knife, is remarkable for the treatment for human prolactinoma. Objective and hypothesis : To clarify the hormonal and pathological effects of single high-dose radiotherapy for human prolactinoma, we used rat prolactinoma model. Method : A 5-week-old male Fischer 344 rat was subcutaneously implanted with 5 mg of diethylstilbestrol (DES) Seven weeks later, prolactinoma was induced and the tumor was treated with single high-dose radiotherapy with 10 mm collimator. Four weeks later, the rats were sacrificed, and weight of pituitary gland, serum prolactin(PRL) and Ki-67(MIB-5) labeling index were measured. Results : Of the control group (n=4), weight of pituitary gland was 7.2 mg, serum PRL was 35.8 ng/ml and MIB-5 labeling was 0.59 %. Of the rats with DES implant and without radiotherapy (n=5) weight of pituitary gland was 11.6 mg, serum PRL was 559 ng/ml and MIB-5 labeling was 0.88 %. Of the rats with DES implant and with 16 Gy radiotherapy (n=4), weight of pituitary gland was 11.1 mg, serum PRL was 121 ng/ml and MIB-5 labeling was 0.46 %. Of the rats with DES implant and with 32 Gy radiotherapy (n=5), weight of pituitary gland was 7.7 mg, serum PRL was 16.8 ng/ml and MIB-5 labeling was 0.38 %. Conclusions : Single high-dose radiotherapy is effective in the treatment for prolactinoma, dose-dependently. A dose of 32 Gy is recommended to practical use.

背景：单次大剂量放射治疗，即伽玛刀，是治疗催乳素瘤的一种有效方法。目的和假设：为了阐明单次大剂量放射治疗对人泌乳素瘤的激素和病理学影响，我们使用大鼠泌乳素瘤模型。方法：将己烯雌酚（DES）5 mg皮下植入5周龄雄性Fischer 344大鼠，7周后诱发泌乳素瘤，用10 mm准直器单次大剂量放射治疗肿瘤。4周后处死大鼠，测定垂体重量、血清催乳素（PRL）和Ki-67（MIB-5）标记指数。结果：对照组（n=4）垂体重量为7.2mg，血清PRL为35.8ng/ml，MIB-5标记率为0.59%。DES植入组（n=5）垂体重量为11.6mg，血清PRL为559 ng/ml，MIB-5标记率为0.88%。DES植入+16戈伊放疗组（n=4）垂体重量为11.1 mg，血清PRL为121 ng/ml，MIB-5标记率为0.46%。植入DES并接受32戈伊放疗的大鼠（n=5），垂体重量为7.7 mg，血清PRL为16.8 ng/ml，MIB-5标记率为0.38%。结论：单次大剂量放射治疗泌乳素瘤疗效确切，且呈剂量依赖性。建议实际使用剂量为32戈伊。

项目成果

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Hideaki Koga、Hiroshi Inoue：“伽马刀治疗产生 GH 的肿瘤”立体定向放射治疗。

甲賀 英明, 橋場 康弘: "海綿静脈洞に湿潤した下垂体腺腫に対するガンマナイフ治療後のMRA follow up"日本内分泌学会雑誌. 77. 96-99 (2001)

Hideaki Koga、Yasuhiro Hashiba：“伽马刀治疗浸润海绵窦的垂体腺瘤后的 MRA 随访”日本内分泌学会杂志 77. 96-99 (2001)。