Single high-dose radiotherapy for experimental rat prolactinoma

单次大剂量放疗治疗实验性大鼠泌乳素瘤

基本信息

批准号：
11671353
负责人：
TAKAHASHI Akio
金额：
$ 2.24万
依托单位：
Gunma University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

Background : Single high-dose radiotherapy, i.e. gamma knife, is remarkable for the treatment for human prolactinoma. Objective and hypothesis : To clarify the hormonal and pathological effects of single high-dose radiotherapy for human prolactinoma, we used rat prolactinoma model. Method : A 5-week-old male Fischer 344 rat was subcutaneously implanted with 5 mg of diethylstilbestrol (DES) Seven weeks later, prolactinoma was induced and the tumor was treated with single high-dose radiotherapy with 10 mm collimator. Four weeks later, the rats were sacrificed, and weight of pituitary gland, serum prolactin(PRL) and Ki-67(MIB-5) labeling index were measured. Results : Of the control group (n=4), weight of pituitary gland was 7.2 mg, serum PRL was 35.8 ng/ml and MIB-5 labeling was 0.59 %. Of the rats with DES implant and without radiotherapy (n=5) weight of pituitary gland was 11.6 mg, serum PRL was 559 ng/ml and MIB-5 labeling was 0.88 %. Of the rats with DES implant and with 16 Gy radiotherapy (n=4), weight of pituitary gland was 11.1 mg, serum PRL was 121 ng/ml and MIB-5 labeling was 0.46 %. Of the rats with DES implant and with 32 Gy radiotherapy (n=5), weight of pituitary gland was 7.7 mg, serum PRL was 16.8 ng/ml and MIB-5 labeling was 0.38 %. Conclusions : Single high-dose radiotherapy is effective in the treatment for prolactinoma, dose-dependently. A dose of 32 Gy is recommended to practical use.

背景：单剂量放射疗法，即伽玛刀，对于人催乳素瘤的治疗而言是显着的。客观和假设：为了阐明单剂量放射疗法对人催乳素瘤的激素和病理作用，我们使用了大鼠催乳素瘤模型。方法：七个星期后，将一只5周龄的雄性Fischer 344大鼠皮下植入5 mg的二乙基苯甲酸酯（DES），诱导催乳素瘤，并用10 mm单密码器用单剂量放射疗法治疗肿瘤。四个星期后，处死大鼠，并测量垂体腺，血清催乳素（PRL）和KI-67（MIB-5）标记指数的重量。结果：在对照组（n = 4）中，垂体的重量为7.2 mg，血清PRL为35.8 ng/ml，MIB-5标记为0.59％。垂体腺体的植入术和没有放疗（n = 5）的大鼠的大鼠为11.6 mg，血清PRL为559 ng/ml，MIB-5标记为0.88％。在具有DES植入物和16 Gy放射疗法（n = 4）的大鼠中，垂体的重量为11.1 mg，血清PRL为121 ng/ml，MIB-5标记为0.46％。在具有DES植入物和32 Gy放射疗法（n = 5）的大鼠中，垂体的重量为7.7 mg，血清PRL为16.8 ng/ml，MIB-5标记为0.38％。结论：单剂量放射疗法可有效地治疗催乳素瘤，剂量依赖性。建议将32 Gy剂量用于实际使用。