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The pharmacological suppression and recovery of male fertility potential investigated with BrdU monoclonal antibody

BrdU单克隆抗体对男性生育潜力的药理抑制和恢复研究

基本信息

批准号：
63570748
负责人：
TAKIHARA Hiroshi
金额：
$ 1.02万
依托单位：
Yamaguchi University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

Antifertility effect of Sulfasalazine(SASP) has been reported by many authors, but its mechanism has not been clear, and the target organ has not been detected. Our purpose of this experiment was to study the antifertility effect of both metabolites of SASP(Sulfapyridine and 5-Aminosalicylate) in male rats and to detect the target organ of Sulfapyridine.Nine-week-old male Sprague Dawly rats, assigned at random to 12 groups, were treated with Sulfapyridine(SP) at 250, 125, 62.5, 31.3mg/kg. body wt/day, and 5-Aminosalicylate(5-ASA) at 153, 76.5, 38.3, 19.Omg/kg. body wt/day and distilled water, for a period of 5 weeks in addition to a normal diet. Mean fertility, testicular weight and diameter ot the seminal tubules in each group was compared. There was a dramatical suppression in fertility in the groups with SP 250mg/kg. day and 125mg/kg. day, however, no suppression was observed in the other groups. No suppression was observed in the mean testicular weight and the mean diameter of the seminal tubules in all groups.The concentration of Acetyl-sulfapyridiie(Ac-SP) in the testis, epididymal head and epididymal tail of the rats treated with SP(250mg/kg. for 5 weeks), were investigated by high pressure liquid chromatography.The concentration of AC-SP was confirmed to be highest in the epididymal head and lowest in the testis. These findings strongly support the hypothesis that SP is the metabolite of SASP, suppressing male fertility and that main target organ of SP is epididymis.

柳氮磺胺吡啶（Sulfasalazine，SASP）的抗生育作用已被许多作者报道，但其作用机制尚不清楚，靶器官尚未发现。为研究柳氮磺胺吡啶（Sulfapyridine）和5-氨基水杨酸（5-Aminosalicylic）的两种代谢产物对雄性大鼠的抗生育作用，本实验将9周龄雄性SD大鼠随机分为12组，分别给予250、125、62.5、31.3mg/kg柳氮磺胺吡啶（SP）。体重/天，5-氨基水杨酸（5-阿萨）153，76.5，38.3，19.0mg/kg。体重/天和蒸馏水，持续5周的时间。比较各组的平均生育力、睾丸重量和精小管直径。SP 250 mg/kg组对小鼠的生育力有明显的抑制作用。125mg/kg.然而，在其他组中没有观察到抑制。各组大鼠睾丸重量和精管直径均无明显变化，睾丸、附睾头和附睾尾中乙酰磺胺吡啶（Ac-SP）含量均明显低于对照组（P < 0. 05）。结果表明，AC-SP在附睾头中的含量最高，在睾丸中的含量最低。这些结果有力地支持了SP是SASP的代谢产物，抑制男性生育力和SP的主要靶器官是附睾的假设。