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On the genesis of trophoblastic deseases using androgenetic and triploid eggs in the mouse

利用小鼠雄激素和三倍体卵研究滋养细胞疾病的发生

基本信息

批准号：
63570785
负责人：
HANDO Tamotsu
金额：
$ 1.41万
依托单位：
Kagawa Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

项目摘要

Hydatidiform moles can be separated into two entities, complete and partial, on the basis of cytogenetics and histopathology. Complete moles are androgenetic in origin, whereas partial moles are triploid. Some of complete moles have been observed clinically to change to choriocarcinomas, but the mechanism of these changes have not been known. So, we designed a study to investigate the morphologic change of the androgenetic eggs in the mouse. The androgenetic egg was produced bY enucleation of the female pronucleus from the triploid egg with two male pronuclei. About 30 to 40 percent of eggs fertilized in vitro were triploidy, using sperms preincubated in the culture solution supplemented with 20 percent fetal calf serum. A female pronucleus could be aspirated from triploid egg by using a small-boreglass pipette (5 to 10 mum in outer diameter) controlled by a micromanipulator. About 20 percent of enucleated diploid androgenetic eggs developed to the two cell stage in vitro. Twenty androgenetic eggs were transplanted in the oviduct or under the capsule of the kidney and testis, but the development of the eggs was not observed. Forty triploid eggs were transplanted in the oviduct, but the implantation in the uterus was not observed. The androgenetic and triploid eggs have not developed in our study. But when these eggs are observed to change to choriocarcinoma, the chromosomal abnormalities as a cause of carcinogenesis in trophoblastic disease, like other cancer in general as well, might be looked as important etiological factor.

根据细胞遗传学和组织病理学，葡萄胎可分为完全性和部分性两种。完全痣起源于雄核发育，而部分痣是三倍体。临床上已观察到一些完全性葡萄胎转变为绒毛膜癌，但这些变化的机制尚不清楚。因此，我们设计了一项研究来观察小鼠雄激素生成卵的形态学变化。雄核发育卵是从具有两个雄性原核的三倍体卵中去核雌性原核而产生的。在体外受精的卵子中，大约有30%到40%是三倍体，使用的精子在补充有20%胎牛血清的培养液中预孵育。用显微操作器控制的小口径吸管（外径5~10 μ m）从三倍体卵中吸出雌性原核。约20%的去核二倍体雄核发育卵在体外发育到二细胞期。将20枚雄激素卵移植于输卵管内或肾、睾丸包膜下，未观察到卵的发育。将40枚三倍体卵移植到输卵管内，但未观察到子宫着床。本研究中未发育出雄核发育卵和三倍体卵。但当观察到这些卵子转变为绒毛膜癌时，染色体异常作为滋养细胞疾病致癌的原因，就像其他癌症一样，可能被视为重要的病因。