Study on the involvement of primary afferent neurons in microcirculatory dynamics in oral region

初级传入神经元参与口腔微循环动力学的研究

• 批准号: 63570869
• 负责人: YONEHARA Norifumi
• 金额: $ 1.34万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570869/
• 关键词: Primary afferent neurons; Microcirculatory hemodynamics; Substance P; Laser doppler flowmeter; Oral region; Hind paw; 炎症反応; 疼痛; 一次求心性線維; 血流量

This study was designed to investigate the involvement of primary afferent neurons in microcirculatory hemodynamics by measuring the blood flow of the rat hind paw and lip with a laser Doppler flowmeter. Moreover the amount of substance P (SP) released into subcutaneous perfusates from hind paw was measured. Electrical stimulation of the sciatic and saphenous nerves containing sensory afferent fibers in the hind paw (10V, 2Hz, 1msec duration) for 30sec inauced an increase of SP release with plasma extravasation and an increase of cutaneous blood flow. Plasma extravasation was determined by pontamine sky blue exudation. Electrical stimulation of the mental nerve, sensory nerve supplying lip, (5v-20V, 2Hz, lmsec duration) for 30 sec also led to an increase of lip blood flow. The cutaneous and lip blood flow increases were significantly inhibited by pretreatment with capsaicin or spantide I.a. injection of phentolamine, propranolol, atropine, methysergide or s.c injection of mepyramine plus cimetidine, on the other handy had no effect. These results suggest that through the peripheral release of SP the small-diameter afferent fibers may play an important role in the regulation of microcirculatory hemodynamics.

本研究采用激光多普勒血流仪测量大鼠后爪和唇部血流，探讨初级传入神经元参与微循环血流动力学的机制。并测定后爪皮下灌注液中P物质的释放量。电刺激后爪包含感觉传入纤维的坐骨神经和隐神经（10V, 2Hz， 1msec持续时间）30sec，可引起SP释放增加，伴血浆外渗，皮肤血流量增加。血浆外渗以波胺天蓝渗出测定。用电刺激支配嘴唇的精神神经和感觉神经30秒（5v-20V, 2Hz， lms持续时间）也会导致嘴唇血流量增加。经辣椒素或spantide预处理的酚妥拉明、心得安、阿托品、甲基塞吉特或s.c注射的甲皮拉明加西咪替丁均可显著抑制皮肤和唇部血流量的增加，而另一方面则无影响。提示小直径传入纤维通过外周释放SP可能在微循环血流动力学调节中起重要作用。

项目成果

猪木令三: "発痛作用とパプチド(川)" 内藤記念科学振興財団研究報告集. 2-4 (1988)

Reizo Inoki：“镇痛作用和肽（河）”内藤纪念科学振兴基金会研究报告2-4（1988）。

Inoki, R.: "Pain producing substances" Clinical Neurosci., 7, (1989) 22-25.

Inoki, R.：“产生疼痛的物质”《临床神经科学》，7，(1989) 22-25。

Inoki R.: INRC,Pergamon Press.

Inoki R.：INRC，佩加蒙出版社。

Yonehara, N.: "Distribution of substance P and methionine-enkephalin in salivary glands and effect of chronic morphine treatment on levels of these peptides" Japan J. Pharmacol., 50, (1989) 503-506.

Yonehara, N.：“唾液腺中 P 物质和甲硫氨酸脑啡肽的分布以及长期吗啡治疗对这些肽水平的影响”Japan J. Pharmacol.，50，(1989) 503-506。

Yonehara,N.: "Participation of substasnce P in infammatory responses" Advances in Exp.Medicine and Biology. 247/B. 529-534 (1989)

Yonehara,N.：“P 物质参与炎症反应”实验医学和生物学进展。