Evaluation of Subendocardial Microcirculatory Hemodynamics by High-Speed Needle-Probe CCD Microscope

高速针探针CCD显微镜评价心内膜下微循环血流动力学

• 批准号: 08558103
• 负责人: KAJIYA Fumihiko
• 金额: $ 7.87万
• 依托单位: Kawasaki Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08558103/
• 关键词: high-speed CCD microscopy system; coronary microcirculation; myocardial ischemia; NADH fluorescence; subendocardium; capillary; microheterogeneity; coronary blood flow; 蛍光生体顕微鏡; 高感度CCD; 血流イメージング; 高速度CCD

Goal of this project was to develop a high-speed CCD microscopy system which can be applied to the studies on the evaluation of microvascular behavior and microcirculatory hemodynamics in the subendocardial myocardial layrs which is at greatest risk of ischemia in the heart. We first developed a high speed CCD microscope and evaluated its performance. This system consists of a needle probe, a camera body, a buffer memory and a videocassette recorder. The vascular images focused on the special CCD through a needle-probe are stored on a digital memory every 5 ms (200images/s). A size of visual field at the needle tip is 1.0*0.8 mm^2 and a spatial resolution is about 2mum. The blood flow velocities in the microvessels were visualized by the CCD system using an optically shinning microsphere. This new system successfully visualized flow in myocardial capillary vessels by using microspheres (diameter : 3mum, specific gravity : 1.3) and NADH fluorescence image from the ischemic myocardium. Major findings are as followings : 1) The velocity pattern of microspheres changed dynamically during a cardiac cycle unlike in the capillary vessels of other organs : the velocity waveform was characterized by exclusively diastolic flow with two-peak systolic reverse flow. 2) In response to the decrease in perfusion pressure, subepicardial-and subendocardial arterioles showed reactive dilations. 3) In response to a transient coronary occlusion, ischemic myocardial region as evaluated by the NADH fluorescence showed spatial microheterogeneity and the size of each ischemic region was about several hundred micron meters.

该项目的目标是开发一种高速CCD显微系统，可用于评估心脏缺血风险最大的心内膜下心肌层的微血管行为和微循环血流动力学的研究。我们首先开发了高速 CCD 显微镜并评估了其性能。该系统由探针、相机机身、缓冲存储器和录像机组成。通过针探头聚焦在特殊 CCD 上的血管图像每 5 毫秒（200 个图像/秒）存储在数字存储器中。针尖视野尺寸为1.0*0.8mm^2，空间分辨率约为2mum。 CCD 系统使用光学发光微球体来可视化微血管中的血流速度。该新系统通过使用微球（直径：3mum，比重：1.3）和来自缺血心肌的 NADH 荧光图像，成功地可视化了心肌毛细血管中的血流。主要发现如下： 1）与其他器官的毛细血管不同，微球的速度模式在心动周期期间动态变化：速度波形的特征是仅舒张血流和双峰收缩期反向血流。 2）响应于灌注压的降低，心外膜下和心内膜下小动脉表现出反应性扩张。 3)响应于短暂的冠状动脉闭塞，通过NADH荧光评估的缺血心肌区域显示出空间微观异质性，并且每个缺血区域的大小约为数百微米。

项目成果

Yasuo Ogasawara: "Development of intra-vital tluoresence CCD microscope system for measurement of NADH in a beating heart" Medical & Biological Engineering & Computing. 35・Suppll. 631 (1997)

Yasuo Ogasawara：“用于测量跳动心脏中 NADH 的活体荧光 CCD 显微镜系统的开发”医学与生物工程与计算 35・增刊 631（1997 年）。

Shinji Yamamori: "Developments of needle-lens probe intravital video-microscope -high resolusion and high speed CCD camera system" 1996 IEEE Engineering in Medicine & Biology. (CD-ROM). paper No.722 (1996)

Shinji Yamamori：“针透镜探针活体视频显微镜的发展 - 高分辨率和高速 CCD 摄像系统” 1996 年 IEEE 医学工程

Fumihiko Kajiya: "Cardiac-Vascular Remodeling and Functional Interaction" Springer-Verlag Tokyo, (1997)

Fumihiko Kajiya：“心脏血管重塑和功能相互作用”Springer-Verlag 东京，（1997 年）

小笠原康夫: "高速度CCD生体顕微鏡による実質臓器微小循環の血流-血管動態の可視化" 日本バイオレオロジー学会誌. 11・4. 195-201 (1997)

Yasuo Ogasawara：“使用高速 CCD 生物显微镜可视化实质器官微循环中的血流和血管动力学”日本生物流变学会杂志 11・4（1997 年）。

Yasuo Ogasawara: "A high speed CCD intravital video microsiope - observation and analysis of microcirculation in a beating heart -" Biomedical Engineering - Applications, Basis and Communications. 8-4. 332-335 (1996)

Yasuo Ogasawara：“高速 CCD 活体视频显微镜 - 观察和分析跳动的心脏中的微循环 -”生物医学工程 - 应用、基础和通信。