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Involvement of neurotrophic factors in the development of neuropathic pain evoked by the loose-ligation of peripheral nerves

神经营养因子参与周围神经松动结扎引起的神经性疼痛的发生

基本信息

批准号：
20592183
负责人：
YONEHARA Norifumi
金额：
$ 2.75万
依托单位：
Ohu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2010
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592183/
关键词：
疼痛神経因性疼痛神経栄養因子熱侵害刺激逃避行動求心路遮断性疼痛アロディニア痛覚過敏マイクロダイアリシスグルタミン酸ノルアドレナリン高速クロマトグラフィー電気検出器 von Freyフィラメント高速クロマトグラフィ-電気化学検出器

项目摘要

It has been already known that various neurotrophic factors act on the repair of the damage site for neuronal regeneration following a peripheral nerve injury. In this study, therefore, to investigate whether or not neurotrophic factors affected the development and/or maintenance of such pathological pain states as the hyperalgesia and allodynia observed with peripheral nerve damage, loose-ligation on the left sciatic nerves of mouse were performed. The responses to thermal stimulation were then measured by the Plantar Test. Hypersensitivity to thermal stimulation developed on the ipsilateral side in ligated animals 3 days after surgery and lasted for at least 49 days. The direct application of neurotrophic factors such as oxidized galectin-1, artemin and platelet-derived neurotrophic factor (BDNF) at the nerve-ligation site showed remarkable inhibition against nerve injury-induced thermal hyperalgesia compared with the direct application of vehicle. Particularly, in the oxidized galectin-1 application group, marked analgesic effect was observed 3-28 days after application of this drug, that is, thermal hyperalgesia developed on the 31 days after injury. Unlike such result obtained by the oxidized galectin-1, however, in the group which applied artemin or platelet-derived neurotrophic factor (BDNF), prolongation of withdrawal latency (analgesic effect) was not observed

已经知道，各种神经营养因子作用于周围神经损伤后神经元再生的损伤部位的修复。因此，在本研究中，为了研究神经营养因子是否影响诸如在外周神经损伤时观察到的痛觉过敏和异常性疼痛的病理性疼痛状态的发展和/或维持，对小鼠的左侧坐骨神经进行松结扎。然后通过足底测试测量对热刺激的反应。术后3天结扎动物的同侧出现对热刺激的超敏反应，并持续至少49天。在神经结扎部位直接应用神经营养因子，如氧化半乳糖凝集素-1，artemin和血小板源性神经营养因子（BDNF），与直接应用载体相比，对神经损伤诱导的热痛觉过敏表现出显着的抑制作用。特别地，在氧化半乳糖凝集素-1施用组中，在施用该药物后3-28天观察到显著的镇痛效果，即，在损伤后31天出现热痛觉过敏。然而，与氧化半乳糖凝集素-1获得的结果不同，在应用artemin或血小板源性神经营养因子（BDNF）的组中，未观察到戒断潜伏期的延长（镇痛效果