STUDIES ON THE STEREOTAXIC APPARATUS AND BRAIN ATLAS OF THE HOUSE MUSK SHREW

麝香鼩立体定位仪及脑图谱的研究

• 批准号: 63580037
• 负责人: KITOH Junzoh
• 金额: $ 1.34万
• 依托单位: NAGOYA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63580037/
• 关键词: Stereotaxic apparatus; Insectivore; House musk shrew; Stereotaxic Atlas

The house musk shrew ( Suncus murinus ) has been domesticated by S.Oda and K.Kondo as new laboratory animal, and was named "suncus". It has been revealed that the suncus has various unique characteristics in the central nervous system and endocrinological control systems.The purposes of this project are to contrive a stereotaxic apparatus applicable to the suncus and to decide the stereotaxis coordinate of the suncus brain.Body size of the suncus is similar to those of young rats or mice, but stereotaxic apparatus for small rodents can not be used for suncus, because the suncus has very different anatomical features of head region from rodents:(1) tympanic bulla and external acustic meatus are not bony but membranous or, a part, cartilageous. (2)external acustic pore of the suncus shifts far ventrally. and axis between the pores of both side runs through larynx. Ear-bars contrived to be applicable to the suncus in this study are more thick and short than those used for rodents; apical diameter is 3mm. basal diameter is 5mm and length is 5mm, and resin pad of 10mm diameter is attached to basal part. These ear-bars can fix tightly head of the suncus, and do not put pressure upon larynx from lateral sides.Temporal plane of skull of the suncus is not round but straight on oral-aboral axis. When this temporal plane is kept horizontally by adjustment of teeth-bar, cerebral temporal surface is also kept horizontal. Under above mentioned condition, the vertical transverse profile through ear-bar 0 plane contains caudal end of the longitudinal cerebral fissure and caudal pole of the mamillary body. This plane is used as the transverse 0 plane of stereo- taxic coordinate in this study. Planes which are parallel to the temporal plane and run through ear-bars or teeth-bar can not be used for horizontal standard plane. because those are too ventrally shifted from the brain. Brain surface is used as horizontal 0 plane in this study.

家麝鼠(Suncus Murinus)是小田和近藤驯化的一种新的实验动物，命名为“Suncus”。本项目的目的是设计一种适用于Suncus的立体定位装置，并确定Suncus脑的立体定位坐标。Suncus的身体大小与幼年大鼠或小鼠相似，但小型啮齿动物的立体定位装置不能用于Suncus，因为Suncus的头部解剖特征与啮齿动物有很大的不同：(1)鼓泡和外耳道不是骨性的，而是膜状的或部分软骨的。(2)Suncus外耳孔向后移位。两侧毛孔之间的轴线贯穿喉部。本研究设计的Suncus耳杆比啮齿动物耳杆短、厚，根尖直径为3 mm。基面直径5 mm，长度5 mm，底面附着直径10 mm的树脂垫。这种耳箍可以将耳塞的头部紧紧固定，不会从侧面对喉部施加压力。耳钉的颅骨临时平面不是圆形的，而是在口-胎轴线上呈直线。当通过调整牙杆使该颞面保持水平时，大脑的颞面也保持水平。在上述条件下，通过耳杆0平面的垂直横断面包括大脑纵裂的尾端和乳头体的尾极。本研究以该平面作为立体定位坐标的横向0平面。与时间平面平行且穿过耳杆或齿条的平面不能用作水平标准平面。因为这些都是从大脑转移过来的腹肌。本研究以脑表面作为水平0平面。