Cytorefractometry - a new technique for refractive index tomography of living cells

细胞折射测量法 - 活细胞折射率断层扫描的新技术

• 批准号: DP0451527
• 负责人: A/Prof Andrei Zvyagin
• 金额: $ 51.73万
• 依托单位: Macquarie University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2004
• 资助国家: 澳大利亚
• 起止时间: 2004-01-01 至 2009-12-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0451527
• 关键词: Cytorefractometry; new; technique; refractive; index

An ultrahigh-resolution bifocal optical coherence refractometry is proposed, and will result to micron-scale-resolution refractive index tomography of living cells, termed cytorefractometry. This technique represents an extension from bifocal optical coherence refractometry that has recently shown a remarkable promise for direct, several-frames-per-second, motion-artifact-free determination of refractive index in turbid media, including biological tissue in vivo. We propose to apply our novel technique to study tissue calcification, a serious problem in cardiology, by making use of the refractive index contrast mechanism. Calcification of smooth muscle cells and aorta subdermal implant models will be studied aiming for prevention of calcification-associated pathologies.

提出了一种超高分辨率的双焦光学相干折射率法，并将其用于活细胞的微米级折射率层析成像，称为细胞折射率法。这项技术代表了双焦光学相干折射法的扩展，最近显示出直接、每秒几帧、无运动伪影地确定混浊介质中的折射率的显著前景，包括活体生物组织。我们建议应用我们的新技术来研究组织钙化，这是心脏病学中的一个严重问题，利用折射率对比机制。将研究平滑肌细胞的钙化和主动脉皮下植入模型，旨在预防钙化相关的病理改变。